Gene Expression Profiling of MicroRNAs in Small-Bowel Transplantation Paraffin-Embedded Mucosal Biopsy Tissue

B. Sotolongo, T. Asaoka, E. Island, M. Carreno, V. Delacruz, D. Cova, C. Russo, P. Tryphonopoulos, J. Moon, D. Weppler, A. Tzakis, Phillip Ruiz

Research output: Contribution to journalArticle

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Abstract

Background: The molecular mechanisms and regulation of immune-mediated rejection of organ allografts remains unclear. Recent studies have reported that small non-coding RNAs, microRNAs (miRNAs) play a critical role in the immune system via modulation of transcription and translation. Purpose: We hypothesized that particular miRNAs provide regulation of an ensuing intragraft immune effector response. The aim of our study was to detect miRNAs involved in acute cellular rejection (AR) in human small intestinal allografts. Materials: We examined 12 small intestinal mucosal biopsies (AR, 7 cases, all grade 2 or 3) and non-rejecting (NR) allografts (5 cases, all grade 0) obtained from recipients after small bowel or multivisceral transplantation. RNA was isolated from the formalin-fixed paraffin-embedded (FFPE) biopsy samples and transcribed to cDNA. After preamplification we utilized a PCR based TaqMan Low Density Array (TLDA) containing 365 mature human miRNAs. Relative quantification was done based on pooled normal intestine using a comparative Ct method. Results: We identified 62 miRNA upregulated genes in small bowels with ACR, and 35 were downregulated. Forty-two miRNA genes were upregulated in non-ACR small bowel biopsy samples (grade IND), and 45 were downregulated. The relative fold change ratio of ACR to non-ACR was calculated, and 50 upregulated and 8 downregulated miRNAs were detected as significant. Several interesting miRNAs will be evaluated further from this preliminary study. Our data suggests that intragraft miRNAs are potentially involved in the activation of a host alloimmune response to donor. These miRNAs may serve as targets for appropriate intervention and may be useful to monitor the allograft status.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalTransplantation Proceedings
Volume42
Issue number1
DOIs
StatePublished - Jan 1 2010

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Gene Expression Profiling
MicroRNAs
Paraffin
Mucous Membrane
Transplantation
Biopsy
Allografts
Down-Regulation
Small Untranslated RNA
Formaldehyde
Genes
Intestines
Immune System
Complementary DNA
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Gene Expression Profiling of MicroRNAs in Small-Bowel Transplantation Paraffin-Embedded Mucosal Biopsy Tissue. / Sotolongo, B.; Asaoka, T.; Island, E.; Carreno, M.; Delacruz, V.; Cova, D.; Russo, C.; Tryphonopoulos, P.; Moon, J.; Weppler, D.; Tzakis, A.; Ruiz, Phillip.

In: Transplantation Proceedings, Vol. 42, No. 1, 01.01.2010, p. 62-65.

Research output: Contribution to journalArticle

Sotolongo, B, Asaoka, T, Island, E, Carreno, M, Delacruz, V, Cova, D, Russo, C, Tryphonopoulos, P, Moon, J, Weppler, D, Tzakis, A & Ruiz, P 2010, 'Gene Expression Profiling of MicroRNAs in Small-Bowel Transplantation Paraffin-Embedded Mucosal Biopsy Tissue', Transplantation Proceedings, vol. 42, no. 1, pp. 62-65. https://doi.org/10.1016/j.transproceed.2009.12.018
Sotolongo, B. ; Asaoka, T. ; Island, E. ; Carreno, M. ; Delacruz, V. ; Cova, D. ; Russo, C. ; Tryphonopoulos, P. ; Moon, J. ; Weppler, D. ; Tzakis, A. ; Ruiz, Phillip. / Gene Expression Profiling of MicroRNAs in Small-Bowel Transplantation Paraffin-Embedded Mucosal Biopsy Tissue. In: Transplantation Proceedings. 2010 ; Vol. 42, No. 1. pp. 62-65.
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AU - Cova, D.

AU - Russo, C.

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