Gene expression profiling of malignant mesothelioma cell lines: cDNA array study

Eeva Kettunen, Anna Maria Nissn, Tiina Ollikainen, Matti Taavitsainen, Johanna Tapper, Karin Mattson, Kaija Linnainmaa, Sakari Knuutila, Wael El-Rifai

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.

Original languageEnglish (US)
Pages (from-to)492-496
Number of pages5
JournalInternational Journal of Cancer
Volume91
Issue number4
DOIs
StatePublished - Feb 15 2001

Keywords

  • Gene expression
  • Malignant mesothelioma
  • Mesothelial cell
  • RT-PCR
  • cDNA array

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Gene expression profiling of malignant mesothelioma cell lines: cDNA array study'. Together they form a unique fingerprint.

  • Cite this

    Kettunen, E., Nissn, A. M., Ollikainen, T., Taavitsainen, M., Tapper, J., Mattson, K., Linnainmaa, K., Knuutila, S., & El-Rifai, W. (2001). Gene expression profiling of malignant mesothelioma cell lines: cDNA array study. International Journal of Cancer, 91(4), 492-496. https://doi.org/10.1002/1097-0215(200002)9999:9999<::AID-IJC1094>3.0.CO;2-M