Gene expression profiling of human fibrocytic myeloid-derived suppressor cells (f-MDSCs)

Emilia Maria Cristina Mazza, Alessia Zoso, Susanna Mandruzzato, Vincenzo Bronte, Paolo Serafini, Luca Inverardi, Silvio Bicciato

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Myeloid-derived suppressor cells (MDSCs) have been shown to control self-reactive and anti-graft effector T-cells in autoimmunity and transplantation, but their therapeutic use is limited by their scarce availability in the peripheral blood of tumor-free donors. We isolated and characterized a novel population of myeloid suppressor cells, named fibrocytic MDSC (f-MDSC), which are differentiated from umbilical cord blood (UCB) precursors (Zoso et al., 2014). This MDSC subset promotes regulatory T-cell expansion and induces normoglycemia in a xenogeneic model of type 1 diabetes. Here we describe in details the experimental design and the bioinformatics analyses of the gene expression dataset used to investigate the molecular mechanisms at the base of MDSC tolerogenic and suppressive properties. We also provide an R code to easily access the data and perform the quality controls and basic analyses relevant to this dataset. Raw and pre-processed data are available at Gene Expression Omnibus under accession GSE52376.

Original languageEnglish (US)
Pages (from-to)389-392
Number of pages4
JournalGenomics Data
Volume2
DOIs
StatePublished - 2014

Keywords

  • Microarrays
  • Myeloid cells
  • Transcriptional data

ASJC Scopus subject areas

  • Molecular Medicine
  • Biochemistry
  • Biotechnology
  • Genetics

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