Gene expression in the mammalian cochlea

A study of multiple vector systems

H. Staecker, D. Li, B. W. O'Malley, Thomas R Van De Water

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Successful delivery of genes to the inner ear has been demonstrated using a variety of vectors and animal models. As our understanding of the molecular pathophysiology of hearing and balance disorders increases, the delivery of genes is becoming central to our ability to manipulate the function of the inner ear. This study evaluates the efficacy of gene transfer and the distribution of three different vector types within the inner ear. Adenovirus vectors, herpes virus vectors and liposomes carrying a plasmid with the green fluorescent protein or beta galactosidase marker genes and a CMV promoter were introduced into the inner ear of 3-month-old mice. The temporal bones and brain were then removed from the animals and examined for transgene expression. Distribution of staining in the treated ear was compared with distribution of staining in the contralateral inner ear. Staining for T cell markers was also carried out to determine inner ear immune response to gene transfer. Herpes virus vectors appear to target neurons most efficiently. Liposome vectors were least efficient in terms of gene transfer. Adenovirus vectors accomplished gene transfer to the widest variety of inner ear cells including auditory and vestibular hair cells. Newer generation adenovirus vectors promise less immune reaction and toxicity than traditional vectors and will be useful for both research and future clinical applications.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalActa Oto-Laryngologica
Volume121
Issue number2
DOIs
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

Cochlea
Inner Ear
Gene Expression
Genes
Adenoviridae
Staining and Labeling
Liposomes
Auditory Hair Cells
Vestibular Hair Cells
Hearing Disorders
Viruses
Temporal Bone
beta-Galactosidase
Green Fluorescent Proteins
Transgenes
Ear
Plasmids
Animal Models
T-Lymphocytes
Neurons

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Gene expression in the mammalian cochlea : A study of multiple vector systems. / Staecker, H.; Li, D.; O'Malley, B. W.; Van De Water, Thomas R.

In: Acta Oto-Laryngologica, Vol. 121, No. 2, 01.05.2001, p. 157-163.

Research output: Contribution to journalArticle

Staecker, H, Li, D, O'Malley, BW & Van De Water, TR 2001, 'Gene expression in the mammalian cochlea: A study of multiple vector systems', Acta Oto-Laryngologica, vol. 121, no. 2, pp. 157-163. https://doi.org/10.1080/000164801300043307
Staecker, H. ; Li, D. ; O'Malley, B. W. ; Van De Water, Thomas R. / Gene expression in the mammalian cochlea : A study of multiple vector systems. In: Acta Oto-Laryngologica. 2001 ; Vol. 121, No. 2. pp. 157-163.
@article{35c782b69988481aac949c4d8a91eec7,
title = "Gene expression in the mammalian cochlea: A study of multiple vector systems",
abstract = "Successful delivery of genes to the inner ear has been demonstrated using a variety of vectors and animal models. As our understanding of the molecular pathophysiology of hearing and balance disorders increases, the delivery of genes is becoming central to our ability to manipulate the function of the inner ear. This study evaluates the efficacy of gene transfer and the distribution of three different vector types within the inner ear. Adenovirus vectors, herpes virus vectors and liposomes carrying a plasmid with the green fluorescent protein or beta galactosidase marker genes and a CMV promoter were introduced into the inner ear of 3-month-old mice. The temporal bones and brain were then removed from the animals and examined for transgene expression. Distribution of staining in the treated ear was compared with distribution of staining in the contralateral inner ear. Staining for T cell markers was also carried out to determine inner ear immune response to gene transfer. Herpes virus vectors appear to target neurons most efficiently. Liposome vectors were least efficient in terms of gene transfer. Adenovirus vectors accomplished gene transfer to the widest variety of inner ear cells including auditory and vestibular hair cells. Newer generation adenovirus vectors promise less immune reaction and toxicity than traditional vectors and will be useful for both research and future clinical applications.",
author = "H. Staecker and D. Li and O'Malley, {B. W.} and {Van De Water}, {Thomas R}",
year = "2001",
month = "5",
day = "1",
doi = "10.1080/000164801300043307",
language = "English",
volume = "121",
pages = "157--163",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Gene expression in the mammalian cochlea

T2 - A study of multiple vector systems

AU - Staecker, H.

AU - Li, D.

AU - O'Malley, B. W.

AU - Van De Water, Thomas R

PY - 2001/5/1

Y1 - 2001/5/1

N2 - Successful delivery of genes to the inner ear has been demonstrated using a variety of vectors and animal models. As our understanding of the molecular pathophysiology of hearing and balance disorders increases, the delivery of genes is becoming central to our ability to manipulate the function of the inner ear. This study evaluates the efficacy of gene transfer and the distribution of three different vector types within the inner ear. Adenovirus vectors, herpes virus vectors and liposomes carrying a plasmid with the green fluorescent protein or beta galactosidase marker genes and a CMV promoter were introduced into the inner ear of 3-month-old mice. The temporal bones and brain were then removed from the animals and examined for transgene expression. Distribution of staining in the treated ear was compared with distribution of staining in the contralateral inner ear. Staining for T cell markers was also carried out to determine inner ear immune response to gene transfer. Herpes virus vectors appear to target neurons most efficiently. Liposome vectors were least efficient in terms of gene transfer. Adenovirus vectors accomplished gene transfer to the widest variety of inner ear cells including auditory and vestibular hair cells. Newer generation adenovirus vectors promise less immune reaction and toxicity than traditional vectors and will be useful for both research and future clinical applications.

AB - Successful delivery of genes to the inner ear has been demonstrated using a variety of vectors and animal models. As our understanding of the molecular pathophysiology of hearing and balance disorders increases, the delivery of genes is becoming central to our ability to manipulate the function of the inner ear. This study evaluates the efficacy of gene transfer and the distribution of three different vector types within the inner ear. Adenovirus vectors, herpes virus vectors and liposomes carrying a plasmid with the green fluorescent protein or beta galactosidase marker genes and a CMV promoter were introduced into the inner ear of 3-month-old mice. The temporal bones and brain were then removed from the animals and examined for transgene expression. Distribution of staining in the treated ear was compared with distribution of staining in the contralateral inner ear. Staining for T cell markers was also carried out to determine inner ear immune response to gene transfer. Herpes virus vectors appear to target neurons most efficiently. Liposome vectors were least efficient in terms of gene transfer. Adenovirus vectors accomplished gene transfer to the widest variety of inner ear cells including auditory and vestibular hair cells. Newer generation adenovirus vectors promise less immune reaction and toxicity than traditional vectors and will be useful for both research and future clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=0035064195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035064195&partnerID=8YFLogxK

U2 - 10.1080/000164801300043307

DO - 10.1080/000164801300043307

M3 - Article

VL - 121

SP - 157

EP - 163

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 2

ER -