Gene expression in mice after high efficiency retroviral-mediated gene transfer

M. A. Eglitis, P. Kantoff, Eli Gilboa, W. F. Anderson

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

A retroviral expression vector (N2) containing the selectable gene, neo(R), has been used to determine the optimal conditions for infecting murine hematopoietic progenitor cells at high efficiency. After infected bone marrow cells were introduced into lethally irradiated mice, the presence, stability, and expression of the vector DNA sequences were analyzed either in individual spleen foci 10 days later or in the blood, bone marrow, and spleens of mice 4 months later. When bone marrow cells were cultured in medium containing virus with titers of more than 106 colonyforming units per milliliter in the presence of purified murine interleukin-3, more than 85 percent of the resulting foci contained vector DNA. This proviral vector DNA was intact. Efficient expression of the neo(R) gene was demonstrated in most of the DNA-positive foci examined. The spleens of reconstituted animals (over a long term) contained intact 'vector DNA' and the blood and bone marrow expressed the neo(R) gene in some animals. Thus, a retroviral vector can be used to introduce intact exogenous DNA sequences into hematopoietic stem cells with high efficiency and with substantial expression.

Original languageEnglish
Pages (from-to)1395-1398
Number of pages4
JournalScience
Volume230
Issue number4732
StatePublished - Dec 1 1985
Externally publishedYes

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vpr Genes
Gene Expression
Spleen
DNA
Hematopoietic Stem Cells
Bone Marrow Cells
Genes
Bone Marrow
Interleukin-3
Viral Load

ASJC Scopus subject areas

  • General

Cite this

Eglitis, M. A., Kantoff, P., Gilboa, E., & Anderson, W. F. (1985). Gene expression in mice after high efficiency retroviral-mediated gene transfer. Science, 230(4732), 1395-1398.

Gene expression in mice after high efficiency retroviral-mediated gene transfer. / Eglitis, M. A.; Kantoff, P.; Gilboa, Eli; Anderson, W. F.

In: Science, Vol. 230, No. 4732, 01.12.1985, p. 1395-1398.

Research output: Contribution to journalArticle

Eglitis, MA, Kantoff, P, Gilboa, E & Anderson, WF 1985, 'Gene expression in mice after high efficiency retroviral-mediated gene transfer', Science, vol. 230, no. 4732, pp. 1395-1398.
Eglitis MA, Kantoff P, Gilboa E, Anderson WF. Gene expression in mice after high efficiency retroviral-mediated gene transfer. Science. 1985 Dec 1;230(4732):1395-1398.
Eglitis, M. A. ; Kantoff, P. ; Gilboa, Eli ; Anderson, W. F. / Gene expression in mice after high efficiency retroviral-mediated gene transfer. In: Science. 1985 ; Vol. 230, No. 4732. pp. 1395-1398.
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