Gene discovery through genomic sequencing of brucella abortus

D. O. Sánchez, R. O. Zandomeni, S. Cravero, Ramiro E Verdun, E. Pierrou, P. Faccio, G. Diaz, S. Lanzavecchia, F. Agüero, A. C C Frasch, S. G E Andersson, O. L. Rossetti, O. Grau, R. A. Ugalde

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Brucella abortus is the etiological agent of brucellosis, a disease that affects bovines and human. We generated DNA random sequences from the genome of B. abortus strain 2308 in order to characterize molecular targets that might be useful for developing immunological or chemotherapeutic strategies against this pathogen. The partial sequencing of 1,899 clones allowed the identification of 1,199 genomic sequence surveys (GSSs) with high homology (BLAST expect value < 10-5) to sequences deposited in the GenBank databases. Among them, 925 represent putative novel genes for the Brucella genus. Out of 925 nonredundant GSSs, 470 were classified in 15 categories based on cellular function. Seven hundred GSSs showed no significant database matches and remain available for further studies in order to identify their function. A high number of GSSs with homology to Agrobacterium tumefaciens and Rhizobium meliloti proteins were observed, thus confirming their close phylogenetic relationship. Among them, several GSSs showed high similarity with genes related to nodule nitrogen fixation, synthesis of nod factors, nodulation protein symbiotic plasmid, and nodule bacteroid differentiation. We have also identified several B. abortus homologs of virulence and pathogenesis genes from other pathogens, including a homolog to both the Shda gene from Salmonella enterica serovar Typhimurium and the AidA-1 gene from Escherichia coli. Other GSSs displayed significant homologies to genes encoding components of the type III and type IV secretion machineries, suggesting that Brucella might also have an active type III secretion machinery.

Original languageEnglish
Pages (from-to)865-868
Number of pages4
JournalInfection and Immunity
Volume69
Issue number2
DOIs
StatePublished - Feb 12 2001
Externally publishedYes

Fingerprint

Brucella abortus
Genetic Association Studies
Brucella
Genes
Databases
Cattle Diseases
Gene Components
Sinorhizobium meliloti
Agrobacterium tumefaciens
Nitrogen Fixation
Salmonella enterica
Brucellosis
Nucleic Acid Databases
Virulence
Surveys and Questionnaires
Proteins
Plasmids
Clone Cells
Genome
Escherichia coli

ASJC Scopus subject areas

  • Immunology

Cite this

Sánchez, D. O., Zandomeni, R. O., Cravero, S., Verdun, R. E., Pierrou, E., Faccio, P., ... Ugalde, R. A. (2001). Gene discovery through genomic sequencing of brucella abortus. Infection and Immunity, 69(2), 865-868. https://doi.org/10.1128/IAI.69.2.865-868.2001

Gene discovery through genomic sequencing of brucella abortus. / Sánchez, D. O.; Zandomeni, R. O.; Cravero, S.; Verdun, Ramiro E; Pierrou, E.; Faccio, P.; Diaz, G.; Lanzavecchia, S.; Agüero, F.; Frasch, A. C C; Andersson, S. G E; Rossetti, O. L.; Grau, O.; Ugalde, R. A.

In: Infection and Immunity, Vol. 69, No. 2, 12.02.2001, p. 865-868.

Research output: Contribution to journalArticle

Sánchez, DO, Zandomeni, RO, Cravero, S, Verdun, RE, Pierrou, E, Faccio, P, Diaz, G, Lanzavecchia, S, Agüero, F, Frasch, ACC, Andersson, SGE, Rossetti, OL, Grau, O & Ugalde, RA 2001, 'Gene discovery through genomic sequencing of brucella abortus', Infection and Immunity, vol. 69, no. 2, pp. 865-868. https://doi.org/10.1128/IAI.69.2.865-868.2001
Sánchez DO, Zandomeni RO, Cravero S, Verdun RE, Pierrou E, Faccio P et al. Gene discovery through genomic sequencing of brucella abortus. Infection and Immunity. 2001 Feb 12;69(2):865-868. https://doi.org/10.1128/IAI.69.2.865-868.2001
Sánchez, D. O. ; Zandomeni, R. O. ; Cravero, S. ; Verdun, Ramiro E ; Pierrou, E. ; Faccio, P. ; Diaz, G. ; Lanzavecchia, S. ; Agüero, F. ; Frasch, A. C C ; Andersson, S. G E ; Rossetti, O. L. ; Grau, O. ; Ugalde, R. A. / Gene discovery through genomic sequencing of brucella abortus. In: Infection and Immunity. 2001 ; Vol. 69, No. 2. pp. 865-868.
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