Gene assignment, expression, and homology of human tropomodulin

Lanping Amy Sung, Yao-Shan Fan, C. C. Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tropomodulin is a newly characterized pointed end capping protein for actin filaments. It binds specifically to the N terminus of tropomyosin and blocks the elongation and depolymerization of tropomyosin-coated actin filaments. A 1.9-kb human tropomodulin cDNA clone was used to map its gene by fluorescence in situ hybridization. The tropomodulin gene was assigned to human chromosome 9q22.2-q22.3, a region that is also known to contain several other genes and disease loci and is proximal to the loci for gelsolin and α- fodrin. The gene for tropomodulin is expressed in major human tissues at different levels in the following order: heart and skeletal muscle much greater than that in brain, lung, and pancreas, which is greater than that in placenta, liver, and kidney. Human tropomodulin and a 64-kDa autoantigen in Graves disease (1D) are related: tropomodulin has 42 and 41% identity with the Graves protein in the N-terminal (69 residue) and C-terminal (194 residue) regions, respectively. The insertion of several homologous repeats in the midsection of the Graves protein, together with the extension of a proline-rich C terminus, accounts for the differences in length between the Graves protein (572 residues) and tropomodulin (359 residues). The significant sequence identity indicates that these two genes are evolved from a common ancestral gene.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalGenomics
Volume34
Issue number1
DOIs
StatePublished - May 15 1996
Externally publishedYes

Fingerprint

Tropomodulin
Gene Expression
Genes
Tropomyosin
Actin Capping Proteins
Gelsolin
Proteins
Human Chromosomes
Fluorescence In Situ Hybridization
Actin Cytoskeleton
Proline
Placenta
Pancreas
Myocardium
Skeletal Muscle
Complementary DNA
Clone Cells
Kidney
Lung

ASJC Scopus subject areas

  • Genetics

Cite this

Gene assignment, expression, and homology of human tropomodulin. / Sung, Lanping Amy; Fan, Yao-Shan; Lin, C. C.

In: Genomics, Vol. 34, No. 1, 15.05.1996, p. 92-96.

Research output: Contribution to journalArticle

Sung, Lanping Amy ; Fan, Yao-Shan ; Lin, C. C. / Gene assignment, expression, and homology of human tropomodulin. In: Genomics. 1996 ; Vol. 34, No. 1. pp. 92-96.
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