Gender differences affect blood flow recovery in a mouse model of hindlimb ischemia

Xinzhi Peng, Jinsong Wang, Roberta Soares, Amir H. Najafi, Subeena Sood, Nima Aghili, Lee O. Alderman, Julio A. Panza, James E. Faber, Shenming Wang, Stephen E. Epstein, Mary Susan Burnett

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Blood flow restoration to ischemic tissue is affected by various risk factors. The aim of this study was to examine gender effects on arteriogenesis and angiogenesis in a mouse ischemic hindlimb model. C57BL/6J mice were subjected to unilateral hindlimb ischemia. Flow recovery was less and hindlimb use impairment was greater in females. No gender difference in vessel number was found at baseline, although 7 days postsurgery females had fewer α-smooth muscle actin-positive vessels in the midpoint of the adductor region. Females had higher hindlimb vascular resistance, were less responsive to vasodilators, and were more sensitive to vasoconstrictors postligation. Western blotting showed that females had higher baseline levels of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in the calf, while 7 days postligation males had higher levels of VEGF, eNOS, and phosphorylated vasodilator stimulated phosphoprotein. Females had less angiogenesis in a Matrigel plug assay and less endothelial cell proliferation in vitro. Females have impaired recovery of flow, a finding presumably caused by multiple factors including decreased collateral remodeling, less angiogenesis, impaired vasodilator response, and increased vasoconstrictor activity; our results also suggest the possibility that new collateral formation, from capillaries, is impaired in females.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume300
Issue number6
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Hindlimb
Ischemia
Nitric Oxide Synthase Type III
Vasoconstrictor Agents
Vasodilator Agents
Vascular Endothelial Growth Factor A
Inbred C57BL Mouse
Vascular Resistance
Smooth Muscle
Actins
Endothelial Cells
Western Blotting
Cell Proliferation

Keywords

  • Angiogenesis
  • Arteriogenesis
  • Vasoreactivity

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Gender differences affect blood flow recovery in a mouse model of hindlimb ischemia. / Peng, Xinzhi; Wang, Jinsong; Soares, Roberta; Najafi, Amir H.; Sood, Subeena; Aghili, Nima; Alderman, Lee O.; Panza, Julio A.; Faber, James E.; Wang, Shenming; Epstein, Stephen E.; Burnett, Mary Susan.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 300, No. 6, 01.06.2011.

Research output: Contribution to journalArticle

Peng, X, Wang, J, Soares, R, Najafi, AH, Sood, S, Aghili, N, Alderman, LO, Panza, JA, Faber, JE, Wang, S, Epstein, SE & Burnett, MS 2011, 'Gender differences affect blood flow recovery in a mouse model of hindlimb ischemia', American Journal of Physiology - Heart and Circulatory Physiology, vol. 300, no. 6. https://doi.org/10.1152/ajpheart.00004.2011
Peng, Xinzhi ; Wang, Jinsong ; Soares, Roberta ; Najafi, Amir H. ; Sood, Subeena ; Aghili, Nima ; Alderman, Lee O. ; Panza, Julio A. ; Faber, James E. ; Wang, Shenming ; Epstein, Stephen E. ; Burnett, Mary Susan. / Gender differences affect blood flow recovery in a mouse model of hindlimb ischemia. In: American Journal of Physiology - Heart and Circulatory Physiology. 2011 ; Vol. 300, No. 6.
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