GCP6 binds to intermediate filaments

A novel function of keratins in the organization of microtubules in epithelial cells

Andrea S. Oriolo, Flavia A. Wald, Gisella Canessa, Pedro J Salas

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In simple epithelial cells, attachment of microtubule-organizing centers (MTOCs) to intermediate filaments (IFs) enables their localization to the apical domain. It is released by cyclin-dependent kinase (Cdk)1 phosphorylation. Here, we identified a component of the γ-tubulin ring complex, γ-tubulin complex protein (GCP)6, as a keratin partner in yeast two-hybrid assays. This was validated by binding in vitro of both purified full-length HIS-tagged GCP6 and a GCP6(1397-1819) fragment to keratins, and pull-down with native IFs. Keratin binding was blocked by Cdk1-mediated phosphorylation of GCP6. GCP6 was apical in normal enterocytes but diffuse in K8-null cells. GCP6 knockdown with short hairpin RNAs (shRNAs) in CACO-2 cells resulted in γ-tubulin signal scattered throughout the cytoplasm, microtubules (MTs) in the perinuclear and basal regions, and microtubule-nucleating activity localized deep in the cytoplasm. Expression of a small fragment GCP6(1397-1513) that competes binding to keratins in vitro displaced γ-tubulin from the cytoskeleton and resulted in depolarization of γ-tubulin and changes in the distribution of microtubules and microtubule nucleation sites. Expression of a full-length S1397D mutant in the Cdk1 phosphorylation site delocalized centrosomes. We conclude that GCP6 participates in the attachment of MTOCs to IFs in epithelial cells and is among the factors that determine the peculiar architecture of microtubules in polarized epithelia.

Original languageEnglish
Pages (from-to)781-794
Number of pages14
JournalMolecular Biology of the Cell
Volume18
Issue number3
DOIs
StatePublished - Mar 1 2007

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Intermediate Filaments
Tubulin
Keratins
Microtubules
Epithelial Cells
Microtubule-Organizing Center
Phosphorylation
Cytoplasm
Epithelial Attachment
CDC2 Protein Kinase
Null Lymphocytes
Centrosome
Two-Hybrid System Techniques
Enterocytes
Cytoskeleton
Small Interfering RNA
Epithelium
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

GCP6 binds to intermediate filaments : A novel function of keratins in the organization of microtubules in epithelial cells. / Oriolo, Andrea S.; Wald, Flavia A.; Canessa, Gisella; Salas, Pedro J.

In: Molecular Biology of the Cell, Vol. 18, No. 3, 01.03.2007, p. 781-794.

Research output: Contribution to journalArticle

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