Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin

Pascal Goldschmidt-Clermont, Robert C. Allen, Andre E. Nel, David L. Emerson, Joseph R. Day, Robert M. Galbraith

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Limited proteolysis of G-actin was performed with trypsin and chymotrypsin to compare the binding sites for Gc and DNase. DNase I bound to the N-terminal area corresponding to the major cleavage site on G-actin (residues 62-68) and inhibited proteolysis, but did not bind the 33.5K C-terminal fragment (G-actin33.5) generated. In contrast, Gc did not exert any inhibitory effect upon proteolysis of the intact native G-actin42.0 molecule, although its presence protected G-actin33.5 from further proteolysis. This was shown by gel filtration to be due to the formation of complexes between Gc and G-actin33.5.

Original languageEnglish
Pages (from-to)735-742
Number of pages8
JournalLife Sciences
Volume38
Issue number8
DOIs
StatePublished - Feb 24 1986
Externally publishedYes

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Vitamin D-Binding Protein
Proteolysis
Actins
Deoxyribonucleases
Deoxyribonuclease I
Gel Chromatography
Gels
Binding Sites
Molecules

ASJC Scopus subject areas

  • Pharmacology

Cite this

Goldschmidt-Clermont, P., Allen, R. C., Nel, A. E., Emerson, D. L., Day, J. R., & Galbraith, R. M. (1986). Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin. Life Sciences, 38(8), 735-742. https://doi.org/10.1016/0024-3205(86)90588-6

Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin. / Goldschmidt-Clermont, Pascal; Allen, Robert C.; Nel, Andre E.; Emerson, David L.; Day, Joseph R.; Galbraith, Robert M.

In: Life Sciences, Vol. 38, No. 8, 24.02.1986, p. 735-742.

Research output: Contribution to journalArticle

Goldschmidt-Clermont, P, Allen, RC, Nel, AE, Emerson, DL, Day, JR & Galbraith, RM 1986, 'Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin', Life Sciences, vol. 38, no. 8, pp. 735-742. https://doi.org/10.1016/0024-3205(86)90588-6
Goldschmidt-Clermont P, Allen RC, Nel AE, Emerson DL, Day JR, Galbraith RM. Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin. Life Sciences. 1986 Feb 24;38(8):735-742. https://doi.org/10.1016/0024-3205(86)90588-6
Goldschmidt-Clermont, Pascal ; Allen, Robert C. ; Nel, Andre E. ; Emerson, David L. ; Day, Joseph R. ; Galbraith, Robert M. / Gc (vitamin D-binding protein) binds the 33.5 K tryptic fragment of actin. In: Life Sciences. 1986 ; Vol. 38, No. 8. pp. 735-742.
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