GB virus C/hepatitis G virus groups and subgroups: Classification by a restriction fragment length polymorphism method based on phylogenetic analysis of the 5' untranslated region

J. F. Quarleri, V. L. Mathet, M. Feld, D. Ferrario, M. P. Della Latta, Ramiro E Verdun, D. O. Sánchez, J. R. Oubiña

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A phylogenetic tree based on 150 5' untranslated region sequences deposited in GenBank database allowed segregation of the sequences into three major groups, including two subgroups, i.e., 1, 2a, 2b, and 3, supported by bootstrap analysis. Restriction site analysis of these sequences predicted that HinfI and either AatII or AciI could be used for genomic typing with 99.4% accuracy. cDNA sequencing and subsequent alignment of 21 Argentine GB virus C/hepatitis G virus strains confirmed restriction fragment length polymorphism patterns theoretically predicted. This method may be useful for a rapid screening of samples when either epidemiological or transmission studies of this agent are carried out.

Original languageEnglish
Pages (from-to)1340-1347
Number of pages8
JournalJournal of Clinical Microbiology
Volume37
Issue number5
StatePublished - Apr 29 1999
Externally publishedYes

Fingerprint

GB virus C
5' Untranslated Regions
Restriction Fragment Length Polymorphisms
Nucleic Acid Databases
Sequence Analysis
Complementary DNA
Databases

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

GB virus C/hepatitis G virus groups and subgroups : Classification by a restriction fragment length polymorphism method based on phylogenetic analysis of the 5' untranslated region. / Quarleri, J. F.; Mathet, V. L.; Feld, M.; Ferrario, D.; Della Latta, M. P.; Verdun, Ramiro E; Sánchez, D. O.; Oubiña, J. R.

In: Journal of Clinical Microbiology, Vol. 37, No. 5, 29.04.1999, p. 1340-1347.

Research output: Contribution to journalArticle

Quarleri, J. F. ; Mathet, V. L. ; Feld, M. ; Ferrario, D. ; Della Latta, M. P. ; Verdun, Ramiro E ; Sánchez, D. O. ; Oubiña, J. R. / GB virus C/hepatitis G virus groups and subgroups : Classification by a restriction fragment length polymorphism method based on phylogenetic analysis of the 5' untranslated region. In: Journal of Clinical Microbiology. 1999 ; Vol. 37, No. 5. pp. 1340-1347.
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