Gating of the designed trimeric/tetrameric voltage-gated H+ channel

Yuichiro Fujiwara, Tatsuki Kurokawa, Kohei Takeshita, Atsushi Nakagawa, H. Peter Larsson, Yasushi Okamura

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The voltage-gated H+ channel functions as a dimer, a configuration that is different from standard tetrameric voltage-gated channels. Each channel protomer has its own permeation pathway. The C-terminal coiled-coil domain has been shown to be necessary for both dimerization and cooperative gating in the two channel protomers. Here we report the gating cooperativity in trimeric and tetrameric Hv channels engineered by altering the hydrophobic core sequence of the coiled-coil assembly domain. Trimeric and tetrameric channels exhibited more rapid and less sigmoidal kinetics of activation of H+ permeation than dimeric channels, suggesting that some channel protomers in trimers and tetramers failed to produce gating cooperativity observed in wild-type dimers. Multimerization of trimer and tetramer channels were confirmed by the biochemical analysis of proteins, including crystallography. These findings indicate that the voltage-gated H+ channel is optimally designed as a dimeric channel on a solid foundation of the sequence pattern of the coiled-coil core, with efficient cooperative gating that ensures sustained and steep voltage-dependent H+ conductance in blood cells.

Original languageEnglish (US)
Pages (from-to)627-640
Number of pages14
JournalJournal of Physiology
Volume591
Issue number3
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Physiology

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    Fujiwara, Y., Kurokawa, T., Takeshita, K., Nakagawa, A., Larsson, H. P., & Okamura, Y. (2013). Gating of the designed trimeric/tetrameric voltage-gated H+ channel. Journal of Physiology, 591(3), 627-640. https://doi.org/10.1113/jphysiol.2012.243006