Gastrointestinal assimilation of Cu during digestion of a single meal in the freshwater rainbow trout (Oncorhynchus mykiss)

Sunita R. Nadella, Carol Bucking, Martin Grosell, Chris M. Wood

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Gastrointestinal processing and assimilation of Cu in vivo was investigated by sequential chyme analysis over a 72 h period following ingestion of a single satiation meal (3% body weight) of commercial trout food (Cu content = 0.42 μmol g- 1) by adult rainbow trout. Leaded glass ballotini beads incorporated into the food and detected by X-ray radiography were employed as an inert marker in order to quantify net Cu absorption or secretion in various parts of the tract. Cu concentrations in the supernatant (fluid phase) fell from about 0.06 μmol mL- 1 (63 μM) in the stomach at 2 h to about 0.003 μmol mL- 1 (3 μM) in the posterior intestine at 72 h. Cu concentrations in the solid phase were 10 to 30-fold higher than in the fluid phase, and increased about 4-fold from the stomach at 2 h to the posterior intestine at 72 h. By reference to the inert marker, overall net Cu absorption from the ingested food by 72 h was about 50%. The mid-intestine, and posterior intestine emerged as important sites of net Cu and water absorption and a potential role for the stomach in this process was also indicated. The anterior intestine was a site of large net Cu addition to the chyme, probably due to large net additions of Cu-containing fluids in the form of bile and other secretions in this segment. The results provide valuable information about sites of Cu absorption and realistic concentrations of Cu in chyme fluid for future in vitro mechanistic studies on Cu transport in the trout gastrointestinal tract.

Original languageEnglish
Pages (from-to)394-401
Number of pages8
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume143
Issue number4
DOIs
StatePublished - Aug 1 2006

Fingerprint

Oncorhynchus mykiss
Fresh Water
Intestines
Meals
Digestion
Fluids
Stomach
Trout
Food
X ray radiography
Satiation
Water absorption
Bile
Radiography
Glass
Gastrointestinal Tract
Eating
Body Weight
X-Rays
Processing

Keywords

  • Absorption
  • Ballotini beads
  • Chyme
  • Dietary Cu
  • Fluid phase
  • Gastrointestinal tract
  • Secretion
  • Solid phase
  • Trout

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Gastrointestinal assimilation of Cu during digestion of a single meal in the freshwater rainbow trout (Oncorhynchus mykiss). / Nadella, Sunita R.; Bucking, Carol; Grosell, Martin; Wood, Chris M.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 143, No. 4, 01.08.2006, p. 394-401.

Research output: Contribution to journalArticle

@article{1987afbd5dbf479599bcb0a8d0b198be,
title = "Gastrointestinal assimilation of Cu during digestion of a single meal in the freshwater rainbow trout (Oncorhynchus mykiss)",
abstract = "Gastrointestinal processing and assimilation of Cu in vivo was investigated by sequential chyme analysis over a 72 h period following ingestion of a single satiation meal (3{\%} body weight) of commercial trout food (Cu content = 0.42 μmol g- 1) by adult rainbow trout. Leaded glass ballotini beads incorporated into the food and detected by X-ray radiography were employed as an inert marker in order to quantify net Cu absorption or secretion in various parts of the tract. Cu concentrations in the supernatant (fluid phase) fell from about 0.06 μmol mL- 1 (63 μM) in the stomach at 2 h to about 0.003 μmol mL- 1 (3 μM) in the posterior intestine at 72 h. Cu concentrations in the solid phase were 10 to 30-fold higher than in the fluid phase, and increased about 4-fold from the stomach at 2 h to the posterior intestine at 72 h. By reference to the inert marker, overall net Cu absorption from the ingested food by 72 h was about 50{\%}. The mid-intestine, and posterior intestine emerged as important sites of net Cu and water absorption and a potential role for the stomach in this process was also indicated. The anterior intestine was a site of large net Cu addition to the chyme, probably due to large net additions of Cu-containing fluids in the form of bile and other secretions in this segment. The results provide valuable information about sites of Cu absorption and realistic concentrations of Cu in chyme fluid for future in vitro mechanistic studies on Cu transport in the trout gastrointestinal tract.",
keywords = "Absorption, Ballotini beads, Chyme, Dietary Cu, Fluid phase, Gastrointestinal tract, Secretion, Solid phase, Trout",
author = "Nadella, {Sunita R.} and Carol Bucking and Martin Grosell and Wood, {Chris M.}",
year = "2006",
month = "8",
day = "1",
doi = "10.1016/j.cbpc.2006.04.012",
language = "English",
volume = "143",
pages = "394--401",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
issn = "1532-0456",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Gastrointestinal assimilation of Cu during digestion of a single meal in the freshwater rainbow trout (Oncorhynchus mykiss)

AU - Nadella, Sunita R.

AU - Bucking, Carol

AU - Grosell, Martin

AU - Wood, Chris M.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Gastrointestinal processing and assimilation of Cu in vivo was investigated by sequential chyme analysis over a 72 h period following ingestion of a single satiation meal (3% body weight) of commercial trout food (Cu content = 0.42 μmol g- 1) by adult rainbow trout. Leaded glass ballotini beads incorporated into the food and detected by X-ray radiography were employed as an inert marker in order to quantify net Cu absorption or secretion in various parts of the tract. Cu concentrations in the supernatant (fluid phase) fell from about 0.06 μmol mL- 1 (63 μM) in the stomach at 2 h to about 0.003 μmol mL- 1 (3 μM) in the posterior intestine at 72 h. Cu concentrations in the solid phase were 10 to 30-fold higher than in the fluid phase, and increased about 4-fold from the stomach at 2 h to the posterior intestine at 72 h. By reference to the inert marker, overall net Cu absorption from the ingested food by 72 h was about 50%. The mid-intestine, and posterior intestine emerged as important sites of net Cu and water absorption and a potential role for the stomach in this process was also indicated. The anterior intestine was a site of large net Cu addition to the chyme, probably due to large net additions of Cu-containing fluids in the form of bile and other secretions in this segment. The results provide valuable information about sites of Cu absorption and realistic concentrations of Cu in chyme fluid for future in vitro mechanistic studies on Cu transport in the trout gastrointestinal tract.

AB - Gastrointestinal processing and assimilation of Cu in vivo was investigated by sequential chyme analysis over a 72 h period following ingestion of a single satiation meal (3% body weight) of commercial trout food (Cu content = 0.42 μmol g- 1) by adult rainbow trout. Leaded glass ballotini beads incorporated into the food and detected by X-ray radiography were employed as an inert marker in order to quantify net Cu absorption or secretion in various parts of the tract. Cu concentrations in the supernatant (fluid phase) fell from about 0.06 μmol mL- 1 (63 μM) in the stomach at 2 h to about 0.003 μmol mL- 1 (3 μM) in the posterior intestine at 72 h. Cu concentrations in the solid phase were 10 to 30-fold higher than in the fluid phase, and increased about 4-fold from the stomach at 2 h to the posterior intestine at 72 h. By reference to the inert marker, overall net Cu absorption from the ingested food by 72 h was about 50%. The mid-intestine, and posterior intestine emerged as important sites of net Cu and water absorption and a potential role for the stomach in this process was also indicated. The anterior intestine was a site of large net Cu addition to the chyme, probably due to large net additions of Cu-containing fluids in the form of bile and other secretions in this segment. The results provide valuable information about sites of Cu absorption and realistic concentrations of Cu in chyme fluid for future in vitro mechanistic studies on Cu transport in the trout gastrointestinal tract.

KW - Absorption

KW - Ballotini beads

KW - Chyme

KW - Dietary Cu

KW - Fluid phase

KW - Gastrointestinal tract

KW - Secretion

KW - Solid phase

KW - Trout

UR - http://www.scopus.com/inward/record.url?scp=33746071527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746071527&partnerID=8YFLogxK

U2 - 10.1016/j.cbpc.2006.04.012

DO - 10.1016/j.cbpc.2006.04.012

M3 - Article

C2 - 16765095

AN - SCOPUS:33746071527

VL - 143

SP - 394

EP - 401

JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

SN - 1532-0456

IS - 4

ER -