Gastric cancers overexpress S100A calcium-binding proteins

Wael El-Rifai, Christopher A. Moskaluk, Mohammad Khalouck Abdrabbo, Jeffery Harper, Cynthia Yoshida, Gregory J. Riggins, Henry F. Frierson, Steven M. Powell

Research output: Contribution to journalArticle

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Abstract

Serial analysis of gene expression provides quantitative and comprehensive expression profiling in a given cell population. In our efforts to define the genes overexpressed in carcinoma of the stomach, we performed serial analysis of gene expression analyses on dissected neoplastic and normal gastric epithelia. We identified 91,334 expressed tags, including 26,633 that were unique. The 20 most up-regulated genes (P <0.01) in gastric cancer (GC) compared with normal gastric epithelia included several keratins that are specific for epithelial cells such as keratin 6A, 13, and 17. Interestingly, five calcium-binding proteins (S100A2, S100A7, S100A8, S100A9, and S100A10) were overexpressed. Quantitative realtime PCR on primary GC samples demonstrated overexpression of S100A2 in 18 of 20 tumors (90%). The other calcium-binding proteins were overexpressed in 25-45% of the GC samples that we studied. Our results indicate that S100A proteins may be important for gastric tumorigenesis. Additional investigations are required to elucidate the biological role of calcium-binding proteins in cancer.

Original languageEnglish (US)
Pages (from-to)6823-6826
Number of pages4
JournalCancer Research
Volume62
Issue number23
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Calcium-Binding Proteins
Stomach Neoplasms
Stomach
Keratin-17
Keratin-13
Epithelium
Keratin-6
Gene Expression
Keratins
Genes
Neoplasms
Carcinogenesis
Epithelial Cells
Carcinoma
Polymerase Chain Reaction
Population
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

El-Rifai, W., Moskaluk, C. A., Abdrabbo, M. K., Harper, J., Yoshida, C., Riggins, G. J., ... Powell, S. M. (2002). Gastric cancers overexpress S100A calcium-binding proteins. Cancer Research, 62(23), 6823-6826.

Gastric cancers overexpress S100A calcium-binding proteins. / El-Rifai, Wael; Moskaluk, Christopher A.; Abdrabbo, Mohammad Khalouck; Harper, Jeffery; Yoshida, Cynthia; Riggins, Gregory J.; Frierson, Henry F.; Powell, Steven M.

In: Cancer Research, Vol. 62, No. 23, 01.12.2002, p. 6823-6826.

Research output: Contribution to journalArticle

El-Rifai, W, Moskaluk, CA, Abdrabbo, MK, Harper, J, Yoshida, C, Riggins, GJ, Frierson, HF & Powell, SM 2002, 'Gastric cancers overexpress S100A calcium-binding proteins', Cancer Research, vol. 62, no. 23, pp. 6823-6826.
El-Rifai W, Moskaluk CA, Abdrabbo MK, Harper J, Yoshida C, Riggins GJ et al. Gastric cancers overexpress S100A calcium-binding proteins. Cancer Research. 2002 Dec 1;62(23):6823-6826.
El-Rifai, Wael ; Moskaluk, Christopher A. ; Abdrabbo, Mohammad Khalouck ; Harper, Jeffery ; Yoshida, Cynthia ; Riggins, Gregory J. ; Frierson, Henry F. ; Powell, Steven M. / Gastric cancers overexpress S100A calcium-binding proteins. In: Cancer Research. 2002 ; Vol. 62, No. 23. pp. 6823-6826.
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