G1 control in mammalian cells.

S. I. Reed, E. Bailly, V. Dulic, L. Hengst, D. Resnitzky, Joyce M Slingerland

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Cyclin-dependent kinases (Cdks) control the major cell cycle transitions in eukaryotic cells. On the basis of a variety of experiments where cyclin function either is impaired or enhanced, D-type cyclins as well as cyclins E and A have been linked to G1 and G1/S phase roles in mammalian cells. We therefore sought to determine if agents that block the G1/S phase transition do so at the level of regulating the Cdk activities associated with these cyclins. A variety of conditions that lead to G1 arrest were found to correlate with accumulation of G1-specific Cdk inhibitors, including treatment of fibroblasts with ionizing radiation, treatment of epithelial cells with TGF-beta, treatment of HeLa cells with the drug lovastatin, and removal of essential growth factors from a variety of different cell types. Mechanistically, inhibition of Cdks was found to involve the stoichiometric binding of Cdk inhibitor proteins. p21Waf1/Cip1 was associated with DNA damage induced arrest while p27Kip1/p28Ick1 accumulated under a variety of antiproliferative conditions.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalJournal of cell science. Supplement
Volume18
StatePublished - Dec 1 1994
Externally publishedYes

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Cyclin-Dependent Kinases
Cyclins
G1 Phase
S Phase
Cyclin D
Cyclin A
Lovastatin
Cyclin E
Phase Transition
Eukaryotic Cells
Cell Cycle Checkpoints
Ionizing Radiation
HeLa Cells
Transforming Growth Factor beta
DNA Damage
Intercellular Signaling Peptides and Proteins
Fibroblasts
Epithelial Cells
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)
  • Cell Biology

Cite this

Reed, S. I., Bailly, E., Dulic, V., Hengst, L., Resnitzky, D., & Slingerland, J. M. (1994). G1 control in mammalian cells. Journal of cell science. Supplement, 18, 69-73.

G1 control in mammalian cells. / Reed, S. I.; Bailly, E.; Dulic, V.; Hengst, L.; Resnitzky, D.; Slingerland, Joyce M.

In: Journal of cell science. Supplement, Vol. 18, 01.12.1994, p. 69-73.

Research output: Contribution to journalArticle

Reed, SI, Bailly, E, Dulic, V, Hengst, L, Resnitzky, D & Slingerland, JM 1994, 'G1 control in mammalian cells.', Journal of cell science. Supplement, vol. 18, pp. 69-73.
Reed SI, Bailly E, Dulic V, Hengst L, Resnitzky D, Slingerland JM. G1 control in mammalian cells. Journal of cell science. Supplement. 1994 Dec 1;18:69-73.
Reed, S. I. ; Bailly, E. ; Dulic, V. ; Hengst, L. ; Resnitzky, D. ; Slingerland, Joyce M. / G1 control in mammalian cells. In: Journal of cell science. Supplement. 1994 ; Vol. 18. pp. 69-73.
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