Gα16, a G protein α subunit specifically expressed in hematopoietic cells

T. T. Amatruda, D. A. Steele, V. Z. Slepak, M. I. Simon

Research output: Contribution to journalArticle

226 Scopus citations

Abstract

Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein α subunit, Gα16, is specifically expressed in human cells of the hematopoietic lineage. The Gα16 cDNA encodes a protein with predicted Mr of 43,500, which resembles the Gq class of α subunits and does not include a pertussis toxin ADP-ribosylation site. In comparison with other G protein α subunits, the Gα16 predicted protein has distinctive amino acid sequences in the amino terminus, the region A guanine nucleotide-binding domain, and in the carboxyl-terminal third of the protein. Cell lines of myelomonocytic and T-cell phenotype express the Gα16 gene, but no expression is detectable in two B-cell lines or in nonhematopoietic cell lines. Gα16 gene expression is downregulated in HL-60 cells induced to differentiate to neutrophils with dimethyl sulfoxide. Antisera generated from synthetic peptides that correspond to two regions of Gα16 specifically react with a protein of 42- to 43-kDa in bacterial strains that overexpress Gα16 and in HL-60 membranes. This protein is decreased in membranes from dimethyl sulfoxide-differentiated HL-60 cells and is not detectable in COS cell membranes. The restricted expression of this gene suggests that Gα16 regulates cell-type-specific signal-transduction pathways, which are not inhibited by pertussis toxin.

Original languageEnglish (US)
Pages (from-to)5587-5591
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number13
DOIs
StatePublished - Sep 4 1991
Externally publishedYes

Keywords

  • Signal transduction

ASJC Scopus subject areas

  • General
  • Genetics

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