Fusion of neurohypophyseal membranes in vitro

Manfred Gratzil, Gerhard Dahl, James T. Russel, Niels A. Thorn

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Freeze cleaving electron microscopy has shown that fusion of isolated secretory vesicles from bovine neurohypophyses was induced by Ca2+ in micromolar concentrations. Mg2+ and Sr2+ were ineffective. Mg2+ inhibited Ca2+-induced fusion. In suspensions containing secretory vesicles as well as sheets of cell membrane, release of vasopressin parallel to intervesicular fusion of secretory vesicles with sheets of cell membrane was observed after exposure to Ca2+. Mg2+ and Sr2+ were ineffective in replacing Ca2+ as trigger for fusion or vasopressin release. Intervesicular fusion and exocytotic profiles were observed when isolated neurohypophyses or neurosecretosome were exposed to cold.

Original languageEnglish (US)
Pages (from-to)45-57
Number of pages13
JournalBBA - Biomembranes
Issue number1
StatePublished - Oct 3 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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