Fusarium keratitis

Genotyping, in vitro susceptibility and clinical outcomes

Rafael A. Oechsler, Michael R. Feilmeier, Darlene Miller, Wei Shi, Ana Luisa Hofling-Lima, Eduardo C Alfonso

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Purpose: To determine differences in the clinical characteristics and antifungal susceptibility patterns among molecularly characterized ocular Fusarium sp isolates. Methods: Fifty-eight isolates of Fusarium sp obtained from 52 eyes of 52 patients were retrieved from the Ocular Microbiology Laboratory of the Bascom Palmer Eye Institute and grown in pure culture. These isolates were characterized based on DNA sequence analysis of the ITS1/2 and ribosomal deoxyribonucleic acid regions. Antifungal susceptibilities were determined for each isolate using broth microdilution methods, and the corresponding medical records were reviewed to determine the clinical outcomes. Results: Fusarium solani isolates had significantly higher values of minimum inhibitory concentration for 90% isolates (MIC90) with voriconazole than F. non-solani organisms (16 and 4 μg/mL, respectively). Isolates of F. solani also exhibited a significantly longer time to cure (65 vs. 40.5 days), a worse follow-up best-corrected visual acuity (20/118 vs. 20/36), and an increased need for urgent surgical management (7 vs. 0 penetrating keratoplasties) when compared with those of F. non-solani. Conclusions: This is the first report to examine the correlation between ocular genotyped Fusarium sp and clinical outcomes. It supports the overall worse prognosis of F. solani versus F. non-solani isolates, including higher voriconazole resistance by the former. The clinical implementation of molecular-based diagnostics and antifungal efficacy testing may yield important prognostic and therapeutic information that could improve the management of fungal ocular infections.

Original languageEnglish
Pages (from-to)667-673
Number of pages7
JournalCornea
Volume32
Issue number5
DOIs
StatePublished - May 1 2013

Fingerprint

Keratitis
Fusarium
Fungal Eye Infections
Penetrating Keratoplasty
Molecular Pathology
Microbial Sensitivity Tests
Microbiology
DNA Sequence Analysis
Visual Acuity
Medical Records
In Vitro Techniques
DNA
Voriconazole

Keywords

  • antifungal
  • clinical outcomes
  • Fusarium sp
  • genotyping
  • keratitis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Fusarium keratitis : Genotyping, in vitro susceptibility and clinical outcomes. / Oechsler, Rafael A.; Feilmeier, Michael R.; Miller, Darlene; Shi, Wei; Hofling-Lima, Ana Luisa; Alfonso, Eduardo C.

In: Cornea, Vol. 32, No. 5, 01.05.2013, p. 667-673.

Research output: Contribution to journalArticle

Oechsler, Rafael A. ; Feilmeier, Michael R. ; Miller, Darlene ; Shi, Wei ; Hofling-Lima, Ana Luisa ; Alfonso, Eduardo C. / Fusarium keratitis : Genotyping, in vitro susceptibility and clinical outcomes. In: Cornea. 2013 ; Vol. 32, No. 5. pp. 667-673.
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abstract = "Purpose: To determine differences in the clinical characteristics and antifungal susceptibility patterns among molecularly characterized ocular Fusarium sp isolates. Methods: Fifty-eight isolates of Fusarium sp obtained from 52 eyes of 52 patients were retrieved from the Ocular Microbiology Laboratory of the Bascom Palmer Eye Institute and grown in pure culture. These isolates were characterized based on DNA sequence analysis of the ITS1/2 and ribosomal deoxyribonucleic acid regions. Antifungal susceptibilities were determined for each isolate using broth microdilution methods, and the corresponding medical records were reviewed to determine the clinical outcomes. Results: Fusarium solani isolates had significantly higher values of minimum inhibitory concentration for 90{\%} isolates (MIC90) with voriconazole than F. non-solani organisms (16 and 4 μg/mL, respectively). Isolates of F. solani also exhibited a significantly longer time to cure (65 vs. 40.5 days), a worse follow-up best-corrected visual acuity (20/118 vs. 20/36), and an increased need for urgent surgical management (7 vs. 0 penetrating keratoplasties) when compared with those of F. non-solani. Conclusions: This is the first report to examine the correlation between ocular genotyped Fusarium sp and clinical outcomes. It supports the overall worse prognosis of F. solani versus F. non-solani isolates, including higher voriconazole resistance by the former. The clinical implementation of molecular-based diagnostics and antifungal efficacy testing may yield important prognostic and therapeutic information that could improve the management of fungal ocular infections.",
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