Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH

Andrew V. Schally; Yoshihiko Baba; Tommie W. Redding; Hisayuki Matsuo; Akira Arimura

doi:10.1159/000122022

Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH

Andrew V. Schally, Yoshihiko Baba, Tommie W. Redding, Hisayuki Matsuo, Akira Arimura

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The cffect of chemical and enzymatic treatments on the biological activity of porcine follicle-stimulating hormone- releasing hormone (FSH-RH) was studied. FSH-RH activity was not affected by trypsin, pepsin, neuraminidase, carboxy- peptidasc A, aminopeptidase M and leucine aminopcptidase. However, incubation with chymotrypsin, subtilisin, papain and pyrrolidonc carboxylyl peptidase abolished the FSH-RI1 activity as did treatment with hydrochloric acid (0.9 m, 1 h, 100°C), diazotized sulfanilic acid, and N-bromosuccinimide. Nitrous acid, sodium mctaperiodate, and ninhydrin did not affect the FSH-RII activity appreciably. Amino acid analyses of the purest FSH-RH preparation showed the following composition: His 1, Arg 1, Scr 1, Glu 1, Pro 1, Gly 2, Leu 1, Trp 1, and Tyr 1. These results and information obtained during purification procedures indicate that a basic polypeptide composed of ten amino acids possesses both LIl-RH and FSII-RH activity.

Original language	English (US)
Pages (from-to)	347-358
Number of pages	12
Journal	Neuroendocrinology
Volume	8
Issue number	6
DOIs	https://doi.org/10.1159/000122022
State	Published - 1971
Externally published	Yes

Keywords

FSH-relcasing hormone
LH-releasing hormone
Polypeptide

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1159/000122022

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title = "Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH",

abstract = "The cffect of chemical and enzymatic treatments on the biological activity of porcine follicle-stimulating hormone- releasing hormone (FSH-RH) was studied. FSH-RH activity was not affected by trypsin, pepsin, neuraminidase, carboxy- peptidasc A, aminopeptidase M and leucine aminopcptidase. However, incubation with chymotrypsin, subtilisin, papain and pyrrolidonc carboxylyl peptidase abolished the FSH-RI1 activity as did treatment with hydrochloric acid (0.9 m, 1 h, 100°C), diazotized sulfanilic acid, and N-bromosuccinimide. Nitrous acid, sodium mctaperiodate, and ninhydrin did not affect the FSH-RII activity appreciably. Amino acid analyses of the purest FSH-RH preparation showed the following composition: His 1, Arg 1, Scr 1, Glu 1, Pro 1, Gly 2, Leu 1, Trp 1, and Tyr 1. These results and information obtained during purification procedures indicate that a basic polypeptide composed of ten amino acids possesses both LIl-RH and FSII-RH activity.",

keywords = "FSH-relcasing hormone, LH-releasing hormone, Polypeptide",

author = "Schally, {Andrew V.} and Yoshihiko Baba and Redding, {Tommie W.} and Hisayuki Matsuo and Akira Arimura",

year = "1971",

doi = "10.1159/000122022",

language = "English (US)",

volume = "8",

pages = "347--358",

journal = "Neuroendocrinology",

issn = "0028-3835",

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TY - JOUR

T1 - Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH

AU - Schally, Andrew V.

AU - Baba, Yoshihiko

AU - Redding, Tommie W.

AU - Matsuo, Hisayuki

AU - Arimura, Akira

PY - 1971

Y1 - 1971

N2 - The cffect of chemical and enzymatic treatments on the biological activity of porcine follicle-stimulating hormone- releasing hormone (FSH-RH) was studied. FSH-RH activity was not affected by trypsin, pepsin, neuraminidase, carboxy- peptidasc A, aminopeptidase M and leucine aminopcptidase. However, incubation with chymotrypsin, subtilisin, papain and pyrrolidonc carboxylyl peptidase abolished the FSH-RI1 activity as did treatment with hydrochloric acid (0.9 m, 1 h, 100°C), diazotized sulfanilic acid, and N-bromosuccinimide. Nitrous acid, sodium mctaperiodate, and ninhydrin did not affect the FSH-RII activity appreciably. Amino acid analyses of the purest FSH-RH preparation showed the following composition: His 1, Arg 1, Scr 1, Glu 1, Pro 1, Gly 2, Leu 1, Trp 1, and Tyr 1. These results and information obtained during purification procedures indicate that a basic polypeptide composed of ten amino acids possesses both LIl-RH and FSII-RH activity.

AB - The cffect of chemical and enzymatic treatments on the biological activity of porcine follicle-stimulating hormone- releasing hormone (FSH-RH) was studied. FSH-RH activity was not affected by trypsin, pepsin, neuraminidase, carboxy- peptidasc A, aminopeptidase M and leucine aminopcptidase. However, incubation with chymotrypsin, subtilisin, papain and pyrrolidonc carboxylyl peptidase abolished the FSH-RI1 activity as did treatment with hydrochloric acid (0.9 m, 1 h, 100°C), diazotized sulfanilic acid, and N-bromosuccinimide. Nitrous acid, sodium mctaperiodate, and ninhydrin did not affect the FSH-RII activity appreciably. Amino acid analyses of the purest FSH-RH preparation showed the following composition: His 1, Arg 1, Scr 1, Glu 1, Pro 1, Gly 2, Leu 1, Trp 1, and Tyr 1. These results and information obtained during purification procedures indicate that a basic polypeptide composed of ten amino acids possesses both LIl-RH and FSII-RH activity.

KW - FSH-relcasing hormone

KW - LH-releasing hormone

KW - Polypeptide

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DO - 10.1159/000122022

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JO - Neuroendocrinology

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SN - 0028-3835

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ER -

Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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