Abstract
Several new HLA-B locus alleles have been discovered in South American Amerindians. By contrast, analysis of the MHC class I alleles of North American native populations has revealed few new HLA-B alleles. This suggests that the HLA-B locus is evolving rapidly in South American populations. Here we describe the HLA-B locus alleles present in individuals from a Central American tribe, the Kuna of Panama. Using a sequence-based typing technique that separates alleles by denaturing gradient gel electrophoresis (DGGE) followed by direct sequencing, we determined the HLA-B alleles from eight Kunas. Two of the HLA-B alleles present in the Kuna have been previously described in other South American Amerindian populations; one allele has been characterized in a Mexican-American. We characterized two new HLA-B alleles in the Kuna, HLA-B(*)3911 and HLA-B(*)5110. HLA-B(*)3911 differed from HLA-B(*)3905 by only a single nucleotide substitution in exon 3. This substitution resulted in an amino acid replacement of leucine by arginine at residue 156 in the alpha 2 domain. Such a change may affect the repertoire of peptides that are bound by this molecule. HLA-B(*)5110 differed significantly from other HLA-B(*)51 alleles in that it is the result of an unusually large intra-locus recombination event of minimally 216 nucleotides. This recombination results in an allele that is part HLA-B(*)51 and part HLA-B(*)40. Thus, more dramatic recombination events may also play a role in the rapid evolution of the HLA-B locus in Amerindians.
| Original language | English |
|---|---|
| Pages (from-to) | 251-257 |
| Number of pages | 7 |
| Journal | Tissue Antigens |
| Volume | 50 |
| Issue number | 3 |
| State | Published - Oct 1 1997 |
| Externally published | Yes |
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Keywords
- DGGE
- HLA-B(*)3911
- HLA-B(*)5110
- Kuna Amerindians
- Sequence typing
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
Further diversification of the HLA-B locus in Central American Amerindians : New B(*)39 and B(*)51 alleles in the Kuna of Panama. / Iwanaga, K. K.; Eberle, M.; Kolman, C. J.; Bermingham, E.; Watkins, David.
In: Tissue Antigens, Vol. 50, No. 3, 01.10.1997, p. 251-257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Further diversification of the HLA-B locus in Central American Amerindians
T2 - New B(*)39 and B(*)51 alleles in the Kuna of Panama
AU - Iwanaga, K. K.
AU - Eberle, M.
AU - Kolman, C. J.
AU - Bermingham, E.
AU - Watkins, David
PY - 1997/10/1
Y1 - 1997/10/1
N2 - Several new HLA-B locus alleles have been discovered in South American Amerindians. By contrast, analysis of the MHC class I alleles of North American native populations has revealed few new HLA-B alleles. This suggests that the HLA-B locus is evolving rapidly in South American populations. Here we describe the HLA-B locus alleles present in individuals from a Central American tribe, the Kuna of Panama. Using a sequence-based typing technique that separates alleles by denaturing gradient gel electrophoresis (DGGE) followed by direct sequencing, we determined the HLA-B alleles from eight Kunas. Two of the HLA-B alleles present in the Kuna have been previously described in other South American Amerindian populations; one allele has been characterized in a Mexican-American. We characterized two new HLA-B alleles in the Kuna, HLA-B(*)3911 and HLA-B(*)5110. HLA-B(*)3911 differed from HLA-B(*)3905 by only a single nucleotide substitution in exon 3. This substitution resulted in an amino acid replacement of leucine by arginine at residue 156 in the alpha 2 domain. Such a change may affect the repertoire of peptides that are bound by this molecule. HLA-B(*)5110 differed significantly from other HLA-B(*)51 alleles in that it is the result of an unusually large intra-locus recombination event of minimally 216 nucleotides. This recombination results in an allele that is part HLA-B(*)51 and part HLA-B(*)40. Thus, more dramatic recombination events may also play a role in the rapid evolution of the HLA-B locus in Amerindians.
AB - Several new HLA-B locus alleles have been discovered in South American Amerindians. By contrast, analysis of the MHC class I alleles of North American native populations has revealed few new HLA-B alleles. This suggests that the HLA-B locus is evolving rapidly in South American populations. Here we describe the HLA-B locus alleles present in individuals from a Central American tribe, the Kuna of Panama. Using a sequence-based typing technique that separates alleles by denaturing gradient gel electrophoresis (DGGE) followed by direct sequencing, we determined the HLA-B alleles from eight Kunas. Two of the HLA-B alleles present in the Kuna have been previously described in other South American Amerindian populations; one allele has been characterized in a Mexican-American. We characterized two new HLA-B alleles in the Kuna, HLA-B(*)3911 and HLA-B(*)5110. HLA-B(*)3911 differed from HLA-B(*)3905 by only a single nucleotide substitution in exon 3. This substitution resulted in an amino acid replacement of leucine by arginine at residue 156 in the alpha 2 domain. Such a change may affect the repertoire of peptides that are bound by this molecule. HLA-B(*)5110 differed significantly from other HLA-B(*)51 alleles in that it is the result of an unusually large intra-locus recombination event of minimally 216 nucleotides. This recombination results in an allele that is part HLA-B(*)51 and part HLA-B(*)40. Thus, more dramatic recombination events may also play a role in the rapid evolution of the HLA-B locus in Amerindians.
KW - DGGE
KW - HLA-B()3911
KW - HLA-B()5110
KW - Kuna Amerindians
KW - Sequence typing
UR - http://www.scopus.com/inward/record.url?scp=0030772161&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030772161&partnerID=8YFLogxK
M3 - Article
C2 - 9331947
AN - SCOPUS:0030772161
VL - 50
SP - 251
EP - 257
JO - HLA
JF - HLA
SN - 2059-2302
IS - 3
ER -