Further diversification of the HLA-B locus in Central American Amerindians: New B(*)39 and B(*)51 alleles in the Kuna of Panama

K. K. Iwanaga, M. Eberle, C. J. Kolman, E. Bermingham, David Watkins

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Several new HLA-B locus alleles have been discovered in South American Amerindians. By contrast, analysis of the MHC class I alleles of North American native populations has revealed few new HLA-B alleles. This suggests that the HLA-B locus is evolving rapidly in South American populations. Here we describe the HLA-B locus alleles present in individuals from a Central American tribe, the Kuna of Panama. Using a sequence-based typing technique that separates alleles by denaturing gradient gel electrophoresis (DGGE) followed by direct sequencing, we determined the HLA-B alleles from eight Kunas. Two of the HLA-B alleles present in the Kuna have been previously described in other South American Amerindian populations; one allele has been characterized in a Mexican-American. We characterized two new HLA-B alleles in the Kuna, HLA-B(*)3911 and HLA-B(*)5110. HLA-B(*)3911 differed from HLA-B(*)3905 by only a single nucleotide substitution in exon 3. This substitution resulted in an amino acid replacement of leucine by arginine at residue 156 in the alpha 2 domain. Such a change may affect the repertoire of peptides that are bound by this molecule. HLA-B(*)5110 differed significantly from other HLA-B(*)51 alleles in that it is the result of an unusually large intra-locus recombination event of minimally 216 nucleotides. This recombination results in an allele that is part HLA-B(*)51 and part HLA-B(*)40. Thus, more dramatic recombination events may also play a role in the rapid evolution of the HLA-B locus in Amerindians.

Original languageEnglish
Pages (from-to)251-257
Number of pages7
JournalTissue Antigens
Volume50
Issue number3
StatePublished - Oct 1 1997
Externally publishedYes

Fingerprint

Panama
HLA-B Antigens
Alleles
Genetic Recombination
Nucleotides
Population
Denaturing Gradient Gel Electrophoresis
North American Indians

Keywords

  • DGGE
  • HLA-B(*)3911
  • HLA-B(*)5110
  • Kuna Amerindians
  • Sequence typing

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Further diversification of the HLA-B locus in Central American Amerindians : New B(*)39 and B(*)51 alleles in the Kuna of Panama. / Iwanaga, K. K.; Eberle, M.; Kolman, C. J.; Bermingham, E.; Watkins, David.

In: Tissue Antigens, Vol. 50, No. 3, 01.10.1997, p. 251-257.

Research output: Contribution to journalArticle

Iwanaga, K. K. ; Eberle, M. ; Kolman, C. J. ; Bermingham, E. ; Watkins, David. / Further diversification of the HLA-B locus in Central American Amerindians : New B(*)39 and B(*)51 alleles in the Kuna of Panama. In: Tissue Antigens. 1997 ; Vol. 50, No. 3. pp. 251-257.
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