Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers

Hong Jiang, Jianguang Zhong, Delia Cabrera DeBuc, Aizhu Tao, Zhe Xu, Byron L Lam, Che Liu, Jianhua Wang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics. Methods: FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60. frames per second and an ~. 210. × high magnification were achieved using the camera inherited high speed setting and Movie Crop Function, for imaging hemodynamics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6. h of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs. Results: The mean bulbar conjunctival vessel diameter was 18.8. ±. 2.7. μm at baseline and increased to 19.6. ±. 2.4. μm after 6. h of lens wear (P = 0.020). The blood flow velocity was increased from 0.60. ±. 0.12. mm/s to 0.88. ±. 0.21. mm/s (P = 0.001). The blood flow rate was also increased from 129.8. ±. 59.9. pl/s to 207.2. ±. 81.3. pl/s (P = 0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63. ±. 0.05 and 1.71. ±. 0.03 analyzed by monofractal and multifractal analyses, respectively. Significant increases in fractal dimensions were found after 6. h of lens wear (P < 0.05). Conclusions: Microvascular network's fractality, morphometry and hemodynamics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodynamics during contact lens wear may indicate ocular microvascular responses to contact lens wear.

Original languageEnglish
Pages (from-to)62-71
Number of pages10
JournalMicrovascular Research
Volume92
DOIs
StatePublished - Jan 1 2014

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Contact lenses
Contact Lenses
Microvessels
Electric lamps
Hemodynamics
Fractals
Perfusion
Imaging techniques
Conjunctiva
Blood
Fractal dimension
Wear of materials
Blood Flow Velocity
Flow velocity
Lenses
Flow rate
High speed cameras
Blood vessels
Digital cameras
Motion Pictures

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers. / Jiang, Hong; Zhong, Jianguang; Cabrera DeBuc, Delia; Tao, Aizhu; Xu, Zhe; Lam, Byron L; Liu, Che; Wang, Jianhua.

In: Microvascular Research, Vol. 92, 01.01.2014, p. 62-71.

Research output: Contribution to journalArticle

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abstract = "Purpose: To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics. Methods: FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60. frames per second and an ~. 210. × high magnification were achieved using the camera inherited high speed setting and Movie Crop Function, for imaging hemodynamics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6. h of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs. Results: The mean bulbar conjunctival vessel diameter was 18.8. ±. 2.7. μm at baseline and increased to 19.6. ±. 2.4. μm after 6. h of lens wear (P = 0.020). The blood flow velocity was increased from 0.60. ±. 0.12. mm/s to 0.88. ±. 0.21. mm/s (P = 0.001). The blood flow rate was also increased from 129.8. ±. 59.9. pl/s to 207.2. ±. 81.3. pl/s (P = 0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63. ±. 0.05 and 1.71. ±. 0.03 analyzed by monofractal and multifractal analyses, respectively. Significant increases in fractal dimensions were found after 6. h of lens wear (P < 0.05). Conclusions: Microvascular network's fractality, morphometry and hemodynamics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodynamics during contact lens wear may indicate ocular microvascular responses to contact lens wear.",
author = "Hong Jiang and Jianguang Zhong and {Cabrera DeBuc}, Delia and Aizhu Tao and Zhe Xu and Lam, {Byron L} and Che Liu and Jianhua Wang",
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T1 - Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers

AU - Jiang, Hong

AU - Zhong, Jianguang

AU - Cabrera DeBuc, Delia

AU - Tao, Aizhu

AU - Xu, Zhe

AU - Lam, Byron L

AU - Liu, Che

AU - Wang, Jianhua

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N2 - Purpose: To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics. Methods: FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60. frames per second and an ~. 210. × high magnification were achieved using the camera inherited high speed setting and Movie Crop Function, for imaging hemodynamics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6. h of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs. Results: The mean bulbar conjunctival vessel diameter was 18.8. ±. 2.7. μm at baseline and increased to 19.6. ±. 2.4. μm after 6. h of lens wear (P = 0.020). The blood flow velocity was increased from 0.60. ±. 0.12. mm/s to 0.88. ±. 0.21. mm/s (P = 0.001). The blood flow rate was also increased from 129.8. ±. 59.9. pl/s to 207.2. ±. 81.3. pl/s (P = 0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63. ±. 0.05 and 1.71. ±. 0.03 analyzed by monofractal and multifractal analyses, respectively. Significant increases in fractal dimensions were found after 6. h of lens wear (P < 0.05). Conclusions: Microvascular network's fractality, morphometry and hemodynamics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodynamics during contact lens wear may indicate ocular microvascular responses to contact lens wear.

AB - Purpose: To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics. Methods: FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60. frames per second and an ~. 210. × high magnification were achieved using the camera inherited high speed setting and Movie Crop Function, for imaging hemodynamics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6. h of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs. Results: The mean bulbar conjunctival vessel diameter was 18.8. ±. 2.7. μm at baseline and increased to 19.6. ±. 2.4. μm after 6. h of lens wear (P = 0.020). The blood flow velocity was increased from 0.60. ±. 0.12. mm/s to 0.88. ±. 0.21. mm/s (P = 0.001). The blood flow rate was also increased from 129.8. ±. 59.9. pl/s to 207.2. ±. 81.3. pl/s (P = 0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63. ±. 0.05 and 1.71. ±. 0.03 analyzed by monofractal and multifractal analyses, respectively. Significant increases in fractal dimensions were found after 6. h of lens wear (P < 0.05). Conclusions: Microvascular network's fractality, morphometry and hemodynamics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodynamics during contact lens wear may indicate ocular microvascular responses to contact lens wear.

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