Functional properties of antibody insulin-like growth factor fusion proteins

Seung Uon Shin, Philip Friden, Marjorie Moran, Sherie L. Morrison

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Genetic engineering and expression techniques have been used to produce antibody growth factor fusion proteins. Insulin-like growth factors (IGFs) 1 and 2 have been fused to mouse-human chimeric IgG3 at the end of C(H)1, immediately after the hinge, and at the end of C(H)3. Fusion heavy chains of the expected molecular weight were expressed, assembled with a co-expressed light chain, and secreted. The resulting molecules continued to bind antigen; they also bound the growth factor receptors, albeit with decreased affinity. The molecule with IGF1 attached after C(H)3 (C(H)3-IGF1) had reduced ability to carry out complement-mediated cytolysis. In contrast the molecule with IGF2 attached after C(H)3 (C(H)3-IGF2) showed an approximately 50-fold increase in its ability to effect complement-mediated cytolysis and so should be an effective cytolytic agent. Both C(H)3-IGF1 and C(H)3-IGF2 bound FcγRI with affinity similar to that of IgG3. The growth factor fusion proteins showed small but significant uptake into the brain parenchyma.

Original languageEnglish (US)
Pages (from-to)4979-4985
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number7
StatePublished - Feb 18 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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