Functional p85α gene is required for normal murine fetal erythropoiesis

Hannah Huddleston, Bailin Tan, Feng-Chun Yang, Hilary White, Mary Jo Wenning, Attilio Orazi, Mervin C. Yoder, Reuben Kapur, David A. Ingram

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In vitro studies suggest that activation of class IA phosphatidyiinositol 3 (PI-3) kinase is necessary for normal erythroid cell development. However, when class IA PI-3 kinase-deficient mice were generated by a targeted deletion of the p85α regulatory subunit, fetal erythropoiesis was reportedly unaffected. Given the discrepancies between these studies, we performed a more detailed in vivo analysis of class IA PI-3 kinase-deficient embryos. Day-14.5 p85α-/- embryos are pale with a marked reduction of mature erythrocytes in their peripheral blood. Further, the absolute number and frequency of both early (erythroid burst-forming unit [BFU-E]) and late erythroid progenitors (erythroid colony-forming unit [CFU-E]) are reduced in p85α-/- fetal livers compared with wild-type controls, which is associated with reduced proliferation. Taken together, these data establish an important role for p85α and class IA PI-3 kinase in regulating the development of both early and late erythroid progenitors in fetal liver.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalBlood
Volume102
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Erythroid Precursor Cells
Erythropoiesis
Phosphotransferases
Genes
Liver
Embryonic Structures
Erythroid Cells
Blood
Erythrocytes
Chemical activation
IA 3

ASJC Scopus subject areas

  • Hematology

Cite this

Huddleston, H., Tan, B., Yang, F-C., White, H., Wenning, M. J., Orazi, A., ... Ingram, D. A. (2003). Functional p85α gene is required for normal murine fetal erythropoiesis. Blood, 102(1), 142-145. https://doi.org/10.1182/blood-2002-10-3245

Functional p85α gene is required for normal murine fetal erythropoiesis. / Huddleston, Hannah; Tan, Bailin; Yang, Feng-Chun; White, Hilary; Wenning, Mary Jo; Orazi, Attilio; Yoder, Mervin C.; Kapur, Reuben; Ingram, David A.

In: Blood, Vol. 102, No. 1, 01.07.2003, p. 142-145.

Research output: Contribution to journalArticle

Huddleston, H, Tan, B, Yang, F-C, White, H, Wenning, MJ, Orazi, A, Yoder, MC, Kapur, R & Ingram, DA 2003, 'Functional p85α gene is required for normal murine fetal erythropoiesis', Blood, vol. 102, no. 1, pp. 142-145. https://doi.org/10.1182/blood-2002-10-3245
Huddleston H, Tan B, Yang F-C, White H, Wenning MJ, Orazi A et al. Functional p85α gene is required for normal murine fetal erythropoiesis. Blood. 2003 Jul 1;102(1):142-145. https://doi.org/10.1182/blood-2002-10-3245
Huddleston, Hannah ; Tan, Bailin ; Yang, Feng-Chun ; White, Hilary ; Wenning, Mary Jo ; Orazi, Attilio ; Yoder, Mervin C. ; Kapur, Reuben ; Ingram, David A. / Functional p85α gene is required for normal murine fetal erythropoiesis. In: Blood. 2003 ; Vol. 102, No. 1. pp. 142-145.
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