Functional micro-imaging of soft and hard tissue using synchrotron light

Philipp J. Thurner, Peter Wyss, Romain Voide, Martin Stauber, Bert Müller, Marco Stampanoni, Jeffrey A. Hubbell, Ralph Müller, Urs Sennhauser

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

In current biological and biomedical research, quantitative endpoints have become an important factor of success. Classically, such endpoints were investigated with 2D imaging, which is usually destructive and the 3D character of tissue gets lost. 3D imaging has gained in importance as a tool for both, qualitative and quantitative assessment of biological systems. In this context synchrotron radiation based tomography has become a very effective tool for opaque 3D tissue systems. Cell cultures and adherent scaffolds are visualized in 3D in a hydrated environment, even uncovering the shape of individual cells. Advanced morphometry allows to characterize the differences between the cell cultures of two distinct phenotypes. Moreover, a new device is presented enabling the 3D investigation of trabecular bone under mechanical load in a time-lapsed fashion. Using the highly brilliant X-rays from a synchrotron radiation source, bone microcracks and an indication for un-cracked ligament bridging are uncovered. 3D microcrack analysis proves that the classification of microcracks from 2D images is ambiguous. Fatigued bone was found to fail in burstlike fashion, whereas non-fatigued bone exhibited a distinct failure band. Additionally, a higher increase in microcrack volume was detected in fatigued in comparison to non-fatigued bone. The developed technologies prove to be very effective tools for advanced 3D imaging of both hard and soft tissue.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsU. Bonse
Pages112-128
Number of pages17
Volume5535
DOIs
StatePublished - 2004
Externally publishedYes
EventDevelopments in X-Ray Tomography IV - Denver, CO, United States
Duration: Aug 4 2004Aug 6 2004

Other

OtherDevelopments in X-Ray Tomography IV
CountryUnited States
CityDenver, CO
Period8/4/048/6/04

Fingerprint

Synchrotrons
bones
microcracks
Microcracks
Bone
synchrotrons
Tissue
Imaging techniques
Synchrotron radiation
Cell culture
synchrotron radiation
ligaments
phenotype
Ligaments
Biological systems
Scaffolds (biology)
radiation sources
Tomography
indication
tomography

Keywords

  • Bone
  • Cell Cultures
  • Contrast Agents
  • Image Guided Failure Assessment
  • Image Processing
  • Imaging
  • Micro-Computed Tomography
  • Microdamage
  • Soft Tissue
  • Synchrotron Radiation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Thurner, P. J., Wyss, P., Voide, R., Stauber, M., Müller, B., Stampanoni, M., ... Sennhauser, U. (2004). Functional micro-imaging of soft and hard tissue using synchrotron light. In U. Bonse (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5535, pp. 112-128). [14] https://doi.org/10.1117/12.559515

Functional micro-imaging of soft and hard tissue using synchrotron light. / Thurner, Philipp J.; Wyss, Peter; Voide, Romain; Stauber, Martin; Müller, Bert; Stampanoni, Marco; Hubbell, Jeffrey A.; Müller, Ralph; Sennhauser, Urs.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / U. Bonse. Vol. 5535 2004. p. 112-128 14.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Thurner, PJ, Wyss, P, Voide, R, Stauber, M, Müller, B, Stampanoni, M, Hubbell, JA, Müller, R & Sennhauser, U 2004, Functional micro-imaging of soft and hard tissue using synchrotron light. in U Bonse (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5535, 14, pp. 112-128, Developments in X-Ray Tomography IV, Denver, CO, United States, 8/4/04. https://doi.org/10.1117/12.559515
Thurner PJ, Wyss P, Voide R, Stauber M, Müller B, Stampanoni M et al. Functional micro-imaging of soft and hard tissue using synchrotron light. In Bonse U, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5535. 2004. p. 112-128. 14 https://doi.org/10.1117/12.559515
Thurner, Philipp J. ; Wyss, Peter ; Voide, Romain ; Stauber, Martin ; Müller, Bert ; Stampanoni, Marco ; Hubbell, Jeffrey A. ; Müller, Ralph ; Sennhauser, Urs. / Functional micro-imaging of soft and hard tissue using synchrotron light. Proceedings of SPIE - The International Society for Optical Engineering. editor / U. Bonse. Vol. 5535 2004. pp. 112-128
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