Functional genomics of physiological plasticity and local adaptation in killifish

Andrew Whitehead, Fernando Galvez, Shujun Zhang, Larissa M. Williams, Marjorie F Oleksiak

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Evolutionary solutions to the physiological challenges of life in highly variable habitats can span the continuum from evolution of a cosmopolitan plastic phenotype to the evolution of locally adapted phenotypes. Killifish (Fundulus sp.) have evolved both highly plastic and locally adapted phenotypes within different selective contexts, providing a comparative system in which to explore the genomic underpinnings of physiological plasticity and adaptive variation. Importantly, extensive variation exists among populations and species for tolerance to a variety of stressors, and we exploit this variation in comparative studies to yield insights into the genomic basis of evolved phenotypic variation. Notably, species of Fundulus occupy the continuum of osmotic habitats from freshwater to marine and populations within Fundulus heteroclitus span far greater variation in pollution tolerance than across all species of fish. Here, we explore how transcriptome regulation underpins extreme physiological plasticity on osmotic shock and how genomic and transcriptomic variation is associated with locally evolved pollution tolerance. We show that F. heteroclitus quickly acclimate to extreme osmotic shock by mounting a dramatic rapid transcriptomic response including an early crisis control phase followed by a tissue remodeling phase involving many regulatory pathways. We also show that convergent evolution of locally adapted pollution tolerance involves complex patterns of gene expression and genome sequence variation, which is confounded with body-weight dependence for some genes. Similarly, exploiting the natural phenotypic variation associated with other established and emerging model organisms is likely to greatly accelerate the pace of discovery of the genomic basis of phenotypic variation. The American Genetic Association. 2010. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.2011

Original languageEnglish
Pages (from-to)499-511
Number of pages13
JournalJournal of Heredity
Volume102
Issue number5
DOIs
StatePublished - Sep 1 2011

Fingerprint

Fundulidae
Genomics
Osmotic Pressure
Phenotype
Plastics
Ecosystem
Fresh Water
Transcriptome
Population
Fishes
Body Weight
Genome
Gene Expression
Genes

Keywords

  • AFLPs
  • comparative transcriptomics
  • Fundulus heteroclitus
  • gene expression
  • microarrays
  • natural populations
  • phenotypic plasticity

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biotechnology
  • Genetics(clinical)

Cite this

Functional genomics of physiological plasticity and local adaptation in killifish. / Whitehead, Andrew; Galvez, Fernando; Zhang, Shujun; Williams, Larissa M.; Oleksiak, Marjorie F.

In: Journal of Heredity, Vol. 102, No. 5, 01.09.2011, p. 499-511.

Research output: Contribution to journalArticle

Whitehead, Andrew ; Galvez, Fernando ; Zhang, Shujun ; Williams, Larissa M. ; Oleksiak, Marjorie F. / Functional genomics of physiological plasticity and local adaptation in killifish. In: Journal of Heredity. 2011 ; Vol. 102, No. 5. pp. 499-511.
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