TY - JOUR
T1 - Functional genomics assays to study CFTR traffic and ENaC function.
AU - Almaça, Joana
AU - Dahimène, Shehrazade
AU - Appel, Nicole
AU - Conrad, Christian
AU - Kunzelmann, Karl
AU - Pepperkok, Rainer
AU - Amaral, Margarida D.
PY - 2011/9/13
Y1 - 2011/9/13
N2 - As several genomes have been sequenced, post-genomic approaches like transcriptomics and proteomics, identifying gene products differentially expressed in association with a given pathology, have held promise both of understanding the pathways associated with the respective disease and as a fast track to therapy. Notwithstanding, these approaches cannot distinguish genes and proteins with mere secondary pathological association from those primarily involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.
AB - As several genomes have been sequenced, post-genomic approaches like transcriptomics and proteomics, identifying gene products differentially expressed in association with a given pathology, have held promise both of understanding the pathways associated with the respective disease and as a fast track to therapy. Notwithstanding, these approaches cannot distinguish genes and proteins with mere secondary pathological association from those primarily involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.
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U2 - 10.1007/978-1-61779-120-8_15
DO - 10.1007/978-1-61779-120-8_15
M3 - Article
C2 - 21547737
AN - SCOPUS:80052553463
VL - 742
SP - 249
EP - 264
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -