Functional genomics assays to study CFTR traffic and ENaC function.

Joana Almaça, Shehrazade Dahimène, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, Margarida D. Amaral

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

As several genomes have been sequenced, post-genomic approaches like transcriptomics and proteomics, identifying gene products differentially expressed in association with a given pathology, have held promise both of understanding the pathways associated with the respective disease and as a fast track to therapy. Notwithstanding, these approaches cannot distinguish genes and proteins with mere secondary pathological association from those primarily involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.

Original languageEnglish (US)
Pages (from-to)249-264
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume742
DOIs
StatePublished - Sep 13 2011
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Almaça, J., Dahimène, S., Appel, N., Conrad, C., Kunzelmann, K., Pepperkok, R., & Amaral, M. D. (2011). Functional genomics assays to study CFTR traffic and ENaC function. Methods in molecular biology (Clifton, N.J.), 742, 249-264. https://doi.org/10.1007/978-1-61779-120-8_15