Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release

S. G. Abdelilah, K. Latifa, N. Esra, L. Cameron, L. Bouchaib, N. C. Nicolaides, Roy C Levitt, Q. Hamid

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Abstract

Human polymorphonuclear neutrophils (PMNs) express surface receptors for various inflammatory mediators, including IgE and IL-4. Recently, the IL-9R locus has been genetically linked to asthma and bronchial hyperresponsiveness in humans. In this study, we evaluated expression of the IL-9R and the effect of IL-9 on human PMNs. RT-PCR analysis showed the presence of IL-9Rαchain mRNA in PMN RNA preparations from asthmatic patients. Using FACS analysis, surface expression of IL-9Rα was detected on PMNs freshly isolated from asthmatics, and to a lesser extent on normal controls. In addition, protein expression of IL-9Rα was also detected in peripheral blood and bronchoalveolar lavage PMNs. Furthermore, functional studies showed that IL-9 stimulation of PMNs results in the release of IL-8 in a concentration-dependent manner. The anti-IL-9 neutralizing Ab suppressed this effect, but had no effect on GM-CSF-induced IL-8 release from PMNs. Taken together, these findings suggest a novel role for PMNs in allergic disease through the expression and activation of the IL-9R.

Original languageEnglish
Pages (from-to)2768-2774
Number of pages7
JournalJournal of Immunology
Volume166
Issue number4
StatePublished - Feb 15 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Abdelilah, S. G., Latifa, K., Esra, N., Cameron, L., Bouchaib, L., Nicolaides, N. C., Levitt, R. C., & Hamid, Q. (2001). Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release. Journal of Immunology, 166(4), 2768-2774.