Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release

S. G. Abdelilah, K. Latifa, N. Esra, L. Cameron, L. Bouchaib, N. C. Nicolaides, Roy C Levitt, Q. Hamid

Research output: Contribution to journalArticle

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Abstract

Human polymorphonuclear neutrophils (PMNs) express surface receptors for various inflammatory mediators, including IgE and IL-4. Recently, the IL-9R locus has been genetically linked to asthma and bronchial hyperresponsiveness in humans. In this study, we evaluated expression of the IL-9R and the effect of IL-9 on human PMNs. RT-PCR analysis showed the presence of IL-9Rαchain mRNA in PMN RNA preparations from asthmatic patients. Using FACS analysis, surface expression of IL-9Rα was detected on PMNs freshly isolated from asthmatics, and to a lesser extent on normal controls. In addition, protein expression of IL-9Rα was also detected in peripheral blood and bronchoalveolar lavage PMNs. Furthermore, functional studies showed that IL-9 stimulation of PMNs results in the release of IL-8 in a concentration-dependent manner. The anti-IL-9 neutralizing Ab suppressed this effect, but had no effect on GM-CSF-induced IL-8 release from PMNs. Taken together, these findings suggest a novel role for PMNs in allergic disease through the expression and activation of the IL-9R.

Original languageEnglish
Pages (from-to)2768-2774
Number of pages7
JournalJournal of Immunology
Volume166
Issue number4
StatePublished - Feb 15 2001
Externally publishedYes

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Interleukin-9 Receptors
Interleukin-8
Neutrophils
Tissue Donors
Interleukin-9
Bronchoalveolar Lavage
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Immunoglobulin E
Asthma

ASJC Scopus subject areas

  • Immunology

Cite this

Abdelilah, S. G., Latifa, K., Esra, N., Cameron, L., Bouchaib, L., Nicolaides, N. C., ... Hamid, Q. (2001). Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release. Journal of Immunology, 166(4), 2768-2774.

Functional expression of IL-9 receptor by human neutrophils from asthmatic donors : Role in IL-8 release. / Abdelilah, S. G.; Latifa, K.; Esra, N.; Cameron, L.; Bouchaib, L.; Nicolaides, N. C.; Levitt, Roy C; Hamid, Q.

In: Journal of Immunology, Vol. 166, No. 4, 15.02.2001, p. 2768-2774.

Research output: Contribution to journalArticle

Abdelilah, SG, Latifa, K, Esra, N, Cameron, L, Bouchaib, L, Nicolaides, NC, Levitt, RC & Hamid, Q 2001, 'Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release', Journal of Immunology, vol. 166, no. 4, pp. 2768-2774.
Abdelilah SG, Latifa K, Esra N, Cameron L, Bouchaib L, Nicolaides NC et al. Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: Role in IL-8 release. Journal of Immunology. 2001 Feb 15;166(4):2768-2774.
Abdelilah, S. G. ; Latifa, K. ; Esra, N. ; Cameron, L. ; Bouchaib, L. ; Nicolaides, N. C. ; Levitt, Roy C ; Hamid, Q. / Functional expression of IL-9 receptor by human neutrophils from asthmatic donors : Role in IL-8 release. In: Journal of Immunology. 2001 ; Vol. 166, No. 4. pp. 2768-2774.
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