Functional constraints of nuclear mitochondrial DNA interactions in xenomitochondrial rodent cell lines

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Abstract

The co-evolution of nuclear and mitochondrial genomes in vertebrates led to more than 100 specific interactions that are crucial for an optimized ATP generation. These interactions have been examined by introducing rat mtDNA into mouse cells devoid of mitochondrial DNA (mtDNA). When mtDNA-less cells derived from the common mouse (Mus musculus domesticus) were fused to cytoplasts prepared from Mus musculus, Mus spretus, or rat (Rattus norvegicus), a comparable number of respiring clones could be obtained. Mouse xenomitochondrial cybrids harboring rat mtDNA had a slower growth rate in medium containing galactose as the carbon soUrce, suggesting a defect in oxidative phosphorylation. These clones respired approximately 56% less than the parental mouse cells or xenomitochondrial cybrids harboring Mus spretus mtDNA. The activities of respiratory complexes I and IV were approximately 56% lower, but mitochondrial protein synthesis was unaffected. The defects in complexes I and IV were associated with decreased steady-state levels of respective subunits suggesting problems in assembly. We also showed that the presence of 10% mouse mtDNA co-existing with rat mtDNA was sufficient to restore respiration to normal levels. Our results suggest that evolutionary distance alone is not a precise predictor of nuclear-mitochondrial interactions as previously suggested for primates.

Original languageEnglish
Pages (from-to)31520-31527
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number40
StatePublished - Oct 6 2000

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Mitochondrial DNA
Rodentia
Cells
Cell Line
Rats
Electron Transport Complex I
Defects
Clone Cells
Mitochondrial Proteins
Galactose
Mitochondrial Genome
Oxidative Phosphorylation
Carbon
Genes
Adenosine Triphosphate
Primates
Vertebrates
Respiration
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

Functional constraints of nuclear mitochondrial DNA interactions in xenomitochondrial rodent cell lines. / Dey, Runu; Barrientos, Antonio; Moraes, Carlos T.

In: Journal of Biological Chemistry, Vol. 275, No. 40, 06.10.2000, p. 31520-31527.

Research output: Contribution to journalArticle

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