Functional consequences of ROMK mutants linked to antenatal Bartter's syndrome and implications for treatment

Ruth A. Schwalbe, Laura Bianchi, Eric A. Accili, Arthur M. Brown

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The antenatal variant of Bartter's syndrome is an autosomal recessive kidney disease characterized by polyhydramnios, premature delivery, hypokalemic alkalosis and hypercalciuria. It is genetically heterogeneous, having been linked recently to mutations in an ATP-sensitive, renal outer medullary K+ channel, ROMK, and earlier to mutations in the Na-K-2Cl co-transporter, NKCC2. We characterized four of the mutations reported in three heterozygous ROMK variants of antenatal Bartter's and found that each expressed a distinct phenotype in Sf9 cells. One mutation expressed normal function and appears to be an allelic polymorphism. The other three mutations produced channels with significantly reduced K+ fluxes. However, the mechanisms in each case were different and reflected abnormalities in phosphorylation, proteolytic processing or protein trafficking. The different mechanisms may be important in the design of appropriate therapy for patients with this disease.

Original languageEnglish
Pages (from-to)975-980
Number of pages6
JournalHuman Molecular Genetics
Volume7
Issue number6
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Bartter Syndrome
Mutation
Symporters
Polyhydramnios
Therapeutics
Sf9 Cells
Hypercalciuria
Alkalosis
Kidney Diseases
Protein Transport
Adenosine Triphosphate
Phosphorylation
Phenotype
Kidney

ASJC Scopus subject areas

  • Genetics

Cite this

Functional consequences of ROMK mutants linked to antenatal Bartter's syndrome and implications for treatment. / Schwalbe, Ruth A.; Bianchi, Laura; Accili, Eric A.; Brown, Arthur M.

In: Human Molecular Genetics, Vol. 7, No. 6, 01.01.1998, p. 975-980.

Research output: Contribution to journalArticle

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