Functional comparison of single- and double-stranded siRNAs in mammalian cells

Yunhe Xu, Annika Linde, Ola Larsson, Dorit Thormeyer, Joacim Elmen, Claes Wahlestedt, Zicai Liang

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The concept of small interfering RNA (siRNA) has been extended to include not only short double-stranded RNA of 19-25bp, but also single-stranded antisense RNA of the same length, since such single-stranded antisense siRNAs were recently found to be able to inhibit gene expression as well. We made comprehensive comparison of double- and single-stranded siRNA functions in RNA interference (RNAi), targeting multiple sites and different mRNAs, measuring RNAi effects at different time-points and in different cell lines, and examining response curves. Duplex siRNAs were found to be more potent than single-stranded antisense siRNAs. This was verified by the observation that single-stranded antisense siRNAs, which were inefficient in some cases when used alone, could be rescued from inefficiency by sequentially transfecting with the sense siRNAs. This result suggests that the structural character of siRNA molecules might be a more important determinant of siRNA efficiency than the cellular persistence of them.

Original languageEnglish (US)
Pages (from-to)680-687
Number of pages8
JournalBiochemical and biophysical research communications
Issue number3
StatePublished - Apr 9 2004
Externally publishedYes


  • CD46
  • Cultured mammalian cells
  • RNA interference
  • Small interfering RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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