Functional characteristics and survival requirements of memory CD4+ T lymphocytes in vivo

Cheryl A. London, Victor L. Perez, Abul K. Abbas

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The phenotypic and functional characteristics of Ag-specific memory CD4+ lymphocytes are poorly defined. To examine the properties and cytokine responsiveness of these cells, we have developed an adoptive transfer system using in vitro-activated T cells expressing the DO.11 transgenic TCR specific for OVA323-339+ I-A(d). In vitro-activated DO.11 CD4+ cells exhibit comparable survival patterns at 1, 6, and 10 wk after adoptive transfer, indicating that a stable population of memory cells has been generated. In the absence of Ag, previously activated T cells survive longer than their naive counterparts in vivo, rapidly revert to a partially naive phenotype, and maintain their effector cytokine profile. The DO.11 CD4+ memory cells are capable of proliferating in response to IL-2 and IL-4, while naive DO.11 CD4+ cells exhibit no such proliferative responses.

Original languageEnglish (US)
Pages (from-to)766-773
Number of pages8
JournalJournal of Immunology
Volume162
Issue number2
StatePublished - Jan 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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