Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection

Joanna M. Bince, Athula Wikramanayake

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The Wnt pathway is a highly conserved signal transduction pathway that plays many critical roles in early animal development. Recent studies have shown that this pathway plays a conserved role in the specification and patterning of the animal - vegetal (A - V) axis in sea urchins and sea anemones. These observations have suggested that the common ancestor to cnidarians and bilaterians used the Wnt signaling pathway for specifying and patterning this maternally established axis. Because the A - V axis plays a critical role in germ layer segregation, a better understanding of how the Wnt pathway is regulated along the A - V axis will provide key insight into the molecular mechanisms regulating germ layer segregation and germ layer evolution in animal embryos. Here, we provide a detailed protocol for using mRNA microinjection that can be used to analyze Wnt signaling in early sea urchin embryos. This protocol can also be adapted to introduce morpholino anti-sense oligonucleotides into sea urchin embryos.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages213-222
Number of pages10
Volume469
DOIs
StatePublished - Dec 1 2008

Publication series

NameMethods in Molecular Biology
Volume469
ISSN (Print)10643745

Fingerprint

Sea Urchins
Microinjections
Embryonic Structures
Germ Layers
Wnt Signaling Pathway
Messenger RNA
Sea Anemones
Cnidaria
Morpholinos
Antisense Oligonucleotides
Signal Transduction

Keywords

  • Animal-vegetal axis
  • Microinjection
  • Sea urchin
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bince, J. M., & Wikramanayake, A. (2008). Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. In Methods in Molecular Biology (Vol. 469, pp. 213-222). (Methods in Molecular Biology; Vol. 469). https://doi.org/10.1007/978-1-60327-469-2_16

Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. / Bince, Joanna M.; Wikramanayake, Athula.

Methods in Molecular Biology. Vol. 469 2008. p. 213-222 (Methods in Molecular Biology; Vol. 469).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bince, JM & Wikramanayake, A 2008, Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. in Methods in Molecular Biology. vol. 469, Methods in Molecular Biology, vol. 469, pp. 213-222. https://doi.org/10.1007/978-1-60327-469-2_16
Bince JM, Wikramanayake A. Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. In Methods in Molecular Biology. Vol. 469. 2008. p. 213-222. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-469-2_16
Bince, Joanna M. ; Wikramanayake, Athula. / Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. Methods in Molecular Biology. Vol. 469 2008. pp. 213-222 (Methods in Molecular Biology).
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