Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits

Tadaaki Miyazaki, Atsuo Kawahara, Hodaka Fujii, Yoko Nakagawa, Yasuhiro Minami, Zhao-Jun Liu, Isao Oishi, Olli Silvennoinen, Bruce A. Witthuhn, James N. Ihle, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

451 Citations (Scopus)

Abstract

The interleukin-2 receptor (IL-2R) consists of three subunits: the IL-2Rα, IL-2Rβ, and IL-2Rγ chains, the last of which is also used in the receptors for IL-4, IL-7, and IL-9. Stimulation with IL-2 induces the tyrosine phosphorylation and activation of the Janus kinases Jak1 and Jak3. Jak1 and Jak3 were found to be selectively associated with the 'serine-rich' region of IL-2Rβ and the carboxyl-terminal region of IL-2Rγ, respectively. Both regions were necessary for IL-2 signaling. Furthermore, Jak3-negative fibroblasts expressing reconstituted IL-2R became responsive to IL-2 after the additional expression of Jak3 complementary DNA. Thus, activation of Jak1 and Jak3 may be a key event in IL-2 signaling.

Original languageEnglish
Pages (from-to)1045-1047
Number of pages3
JournalScience
Volume266
Issue number5187
StatePublished - Nov 11 1994
Externally publishedYes

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Interleukin-2 Receptors
Interleukin-2
Interleukin-9
Interleukin-4 Receptors
Janus Kinases
Interleukin-7
Serine
Complementary DNA
Fibroblasts
Phosphorylation

ASJC Scopus subject areas

  • General

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Miyazaki, T., Kawahara, A., Fujii, H., Nakagawa, Y., Minami, Y., Liu, Z-J., ... Taniguchi, T. (1994). Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science, 266(5187), 1045-1047.

Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. / Miyazaki, Tadaaki; Kawahara, Atsuo; Fujii, Hodaka; Nakagawa, Yoko; Minami, Yasuhiro; Liu, Zhao-Jun; Oishi, Isao; Silvennoinen, Olli; Witthuhn, Bruce A.; Ihle, James N.; Taniguchi, Tadatsugu.

In: Science, Vol. 266, No. 5187, 11.11.1994, p. 1045-1047.

Research output: Contribution to journalArticle

Miyazaki, T, Kawahara, A, Fujii, H, Nakagawa, Y, Minami, Y, Liu, Z-J, Oishi, I, Silvennoinen, O, Witthuhn, BA, Ihle, JN & Taniguchi, T 1994, 'Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits', Science, vol. 266, no. 5187, pp. 1045-1047.
Miyazaki T, Kawahara A, Fujii H, Nakagawa Y, Minami Y, Liu Z-J et al. Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science. 1994 Nov 11;266(5187):1045-1047.
Miyazaki, Tadaaki ; Kawahara, Atsuo ; Fujii, Hodaka ; Nakagawa, Yoko ; Minami, Yasuhiro ; Liu, Zhao-Jun ; Oishi, Isao ; Silvennoinen, Olli ; Witthuhn, Bruce A. ; Ihle, James N. ; Taniguchi, Tadatsugu. / Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. In: Science. 1994 ; Vol. 266, No. 5187. pp. 1045-1047.
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AU - Liu, Zhao-Jun

AU - Oishi, Isao

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