FTIR imaging analysis of bioactive microsphere incorporated stem cell sheets for osteochondral defect repair

Nicholas Caccese, Uday Palukuru, Pooja Thakkar, Cushla McGoverin, Nancy Pleshko, Christina Cheng, Loran Solorio, Diego Correa, Lauren Philips, Alexandra McMillan, Eben Alsberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Several tissue engineering approaches have been proposed for articular cartilage repair, but to date there is no consensus on the optimal method. Self-assembling microsphere-incorporated cell sheets have been developed recently for cartilage tissue engineering applications, as they provide an environment for the chondrogenic induction of human mesenchymal stem cells (hMSCs). In the current study, early articular cartilage repair was assessed in a rabbit osteochondral defect model treated with self-assembling hMSC sheets incorporated with bioactive polymer microspheres. Histological sections were evaluated by FTIR imaging for molecular composition. We found that the incorporation of transforming growth factor β1 to the microspheres improved the quality of repair tissue based on increased repair tissue, and increased collagen maturity, at one and three months post-repair. Evaluation of repair tissue at longer timepoints will assess whether these preliminary results will extend to the quality of more mature repair tissue.

Original languageEnglish (US)
Title of host publicationProceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Volume2014-December
ISBN (Electronic)9781479937288
DOIs
StatePublished - Jan 1 2014
Externally publishedYes
Event2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 - Boston, United States
Duration: Apr 25 2014Apr 27 2014

Other

Other2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
CountryUnited States
CityBoston
Period4/25/144/27/14

Keywords

  • Cartilage repair
  • Collagen
  • Gelatin microspheres
  • Infrared spectroscopy
  • Osteochondral repair

ASJC Scopus subject areas

  • Bioengineering

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