Frictional response of bovine articular cartilage under creep loading following proteoglycan digestion with chondroitinase ABC

Ines M. Basalo, Faye Hui Chen, Clark T. Hung, Gerard A. Ateshian

Research output: Contribution to journalArticle

30 Scopus citations


The specific aim of this study was to investigate the effect of chondroitinase ABC treatment on the frictional response of bovine articular cartilage against glass, under creep loading. The hypothesis is that chondroitinase ABC treatment increases the friction coefficient of bovine articular cartilage under creep. Articular cartilage samples (n=12) harvested from two bovine knee joints (1-3 months old) were divided into a control group (intact specimens) and a treated group (chondroitinase ABC digestion), and tested in unconfined compression with simultaneous continuous sliding (±4 mm at 1 mm/s) under a constant applied stress of 0.5 MPa, for 2500 s. The time-dependent response of the friction coefficient was measured. With increasing duration of loading, treated samples exhibited a significantly higher friction coefficient than control samples as assessed by the equilibrium value (treated: μeq = 0.19±0.02; control: μeq=0. 12±0.03; p =0.002), though the coefficient achieved immediately upon loading did not increase significantly (treated: μmin=0. 0053±0.0025; control: μmin=0.037±0.0013; p=0.19). Our results demonstrate that removal of the cartilage glycosaminoglycans using chondroitinase ABC significantly increases the overall time-dependent friction coefficient of articular cartilage. These findings strengthen the motivation for developing chondroprotective strategies by increasing cartilage chondroitin sulfate content in osteoarthritic joints.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalJournal of biomechanical engineering
Issue number1
StatePublished - Feb 1 2006



  • Cartilage
  • Enzymatic digestion
  • Friction
  • GAG
  • Proteoglycan

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

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