Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB)

Fatoumata Sissoko, Amy Junnila, Mohamad M. Traore, Sekou F. Traore, Seydou Doumbia, Seydou Mamadou Dembele, Yosef Schlein, Amadou Sekou Traore, Petrányi Gergely, Rui De Xue, Kristopher L. Arheart, Edita E. Revay, Vasiliy D. Kravchenko, John C Beier, Gunter C. Müller

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background Current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including Zika, dengue, chikungunya, and yellow fever. Hence there is a growing public health challenge to identify the best new control tools to use against the vector Aedes aegypti. In this study, we investigated Ae. aegypti sugar feeding strategies in Bamako, Mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (ATSB). Methodology We determined the relative attraction of Ae. aegypti males and females to a variety of sugar sources including flowers, fruits, seedpods, and honeydew in the laboratory and using plant-baited traps in the field. Next, we observed the rhythm of blood feeding versus sugar feeding activity of Ae. aegypti in vegetation and in open areas. Finally, we studied the effectiveness of spraying vegetation with ATSB on Ae. aegypti in sugar rich (lush vegetation) and in sugar poor (sparse vegetation) urban environments. Principal findings Male and female laboratory sugar feeding rates within 24 h, on 8 of 16 plants offered were over 80%. The survival rates of mosquitoes on several plant sources were nearly as long as that of controls maintained on sucrose solution. In the field, females were highly attracted to 11 of 20 sugar sources, and 8 of these were attractive to males. Peak periods of host attraction for blood-feeding and sugar feeding in open areas were nearly identical and occurred shortly after sunrise and around sunset. In shaded areas, the first sugar-seeking peak occurred between 11:30 and 12:30 while the second was from 16:30 to 17:30. In a 50-day field trial, ATSB significantly reduced mean numbers of landing / biting female Ae. aegypti in the two types of vegetation. At sugar poor sites, the mean pre-treatment catch of 20.51 females on day 14 was reduced 70-fold to 0.29 on day 50. At sugar rich sites, the mean pretreatment catch of 32.46 females on day 14 was reduced 10-fold to a mean of 3.20 females on day 50. Conclusions This is the first study to show how the vector Ae. aegypti depends on environmental resources of sugar for feeding and survival. The demonstration that Ae. aegypti populations rapidly collapsed after ATSB treatment, in both sugar rich and sugar poor environments, is strong evidence that Ae. aegypti is sugar-feeding frequently. Indeed, this study clearly demonstrates that Ae. aegypti mosquitoes depend on natural sugar resources, and a promising new method for vector control, ATSB, can be highly effective in the fight against Aedes-transmitted diseases.

Original languageEnglish (US)
Article numbere0214170
JournalPloS one
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2019

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sugar feeding
Mali
Aedes
Poisons
Feeding Behavior
Aedes aegypti
Sugars
baits
feeding behavior
sugars
Culicidae
Yellow Fever
Arboviruses
Dengue
vegetation
Disease Outbreaks
Sucrose
Blood Glucose
Fruit
Public Health

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sissoko, F., Junnila, A., Traore, M. M., Traore, S. F., Doumbia, S., Dembele, S. M., ... Müller, G. C. (2019). Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB). PloS one, 14(6), [e0214170]. https://doi.org/10.1371/journal.pone.0214170

Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB). / Sissoko, Fatoumata; Junnila, Amy; Traore, Mohamad M.; Traore, Sekou F.; Doumbia, Seydou; Dembele, Seydou Mamadou; Schlein, Yosef; Traore, Amadou Sekou; Gergely, Petrányi; Xue, Rui De; Arheart, Kristopher L.; Revay, Edita E.; Kravchenko, Vasiliy D.; Beier, John C; Müller, Gunter C.

In: PloS one, Vol. 14, No. 6, e0214170, 01.06.2019.

Research output: Contribution to journalArticle

Sissoko, F, Junnila, A, Traore, MM, Traore, SF, Doumbia, S, Dembele, SM, Schlein, Y, Traore, AS, Gergely, P, Xue, RD, Arheart, KL, Revay, EE, Kravchenko, VD, Beier, JC & Müller, GC 2019, 'Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB)', PloS one, vol. 14, no. 6, e0214170. https://doi.org/10.1371/journal.pone.0214170
Sissoko, Fatoumata ; Junnila, Amy ; Traore, Mohamad M. ; Traore, Sekou F. ; Doumbia, Seydou ; Dembele, Seydou Mamadou ; Schlein, Yosef ; Traore, Amadou Sekou ; Gergely, Petrányi ; Xue, Rui De ; Arheart, Kristopher L. ; Revay, Edita E. ; Kravchenko, Vasiliy D. ; Beier, John C ; Müller, Gunter C. / Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB). In: PloS one. 2019 ; Vol. 14, No. 6.
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abstract = "Background Current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including Zika, dengue, chikungunya, and yellow fever. Hence there is a growing public health challenge to identify the best new control tools to use against the vector Aedes aegypti. In this study, we investigated Ae. aegypti sugar feeding strategies in Bamako, Mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (ATSB). Methodology We determined the relative attraction of Ae. aegypti males and females to a variety of sugar sources including flowers, fruits, seedpods, and honeydew in the laboratory and using plant-baited traps in the field. Next, we observed the rhythm of blood feeding versus sugar feeding activity of Ae. aegypti in vegetation and in open areas. Finally, we studied the effectiveness of spraying vegetation with ATSB on Ae. aegypti in sugar rich (lush vegetation) and in sugar poor (sparse vegetation) urban environments. Principal findings Male and female laboratory sugar feeding rates within 24 h, on 8 of 16 plants offered were over 80{\%}. The survival rates of mosquitoes on several plant sources were nearly as long as that of controls maintained on sucrose solution. In the field, females were highly attracted to 11 of 20 sugar sources, and 8 of these were attractive to males. Peak periods of host attraction for blood-feeding and sugar feeding in open areas were nearly identical and occurred shortly after sunrise and around sunset. In shaded areas, the first sugar-seeking peak occurred between 11:30 and 12:30 while the second was from 16:30 to 17:30. In a 50-day field trial, ATSB significantly reduced mean numbers of landing / biting female Ae. aegypti in the two types of vegetation. At sugar poor sites, the mean pre-treatment catch of 20.51 females on day 14 was reduced 70-fold to 0.29 on day 50. At sugar rich sites, the mean pretreatment catch of 32.46 females on day 14 was reduced 10-fold to a mean of 3.20 females on day 50. Conclusions This is the first study to show how the vector Ae. aegypti depends on environmental resources of sugar for feeding and survival. The demonstration that Ae. aegypti populations rapidly collapsed after ATSB treatment, in both sugar rich and sugar poor environments, is strong evidence that Ae. aegypti is sugar-feeding frequently. Indeed, this study clearly demonstrates that Ae. aegypti mosquitoes depend on natural sugar resources, and a promising new method for vector control, ATSB, can be highly effective in the fight against Aedes-transmitted diseases.",
author = "Fatoumata Sissoko and Amy Junnila and Traore, {Mohamad M.} and Traore, {Sekou F.} and Seydou Doumbia and Dembele, {Seydou Mamadou} and Yosef Schlein and Traore, {Amadou Sekou} and Petr{\'a}nyi Gergely and Xue, {Rui De} and Arheart, {Kristopher L.} and Revay, {Edita E.} and Kravchenko, {Vasiliy D.} and Beier, {John C} and M{\"u}ller, {Gunter C.}",
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T1 - Frequent sugar feeding behavior by Aedes aegypti in Bamako, Mali makes them ideal candidates for control with attractive toxic sugar baits (ATSB)

AU - Sissoko, Fatoumata

AU - Junnila, Amy

AU - Traore, Mohamad M.

AU - Traore, Sekou F.

AU - Doumbia, Seydou

AU - Dembele, Seydou Mamadou

AU - Schlein, Yosef

AU - Traore, Amadou Sekou

AU - Gergely, Petrányi

AU - Xue, Rui De

AU - Arheart, Kristopher L.

AU - Revay, Edita E.

AU - Kravchenko, Vasiliy D.

AU - Beier, John C

AU - Müller, Gunter C.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Background Current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including Zika, dengue, chikungunya, and yellow fever. Hence there is a growing public health challenge to identify the best new control tools to use against the vector Aedes aegypti. In this study, we investigated Ae. aegypti sugar feeding strategies in Bamako, Mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (ATSB). Methodology We determined the relative attraction of Ae. aegypti males and females to a variety of sugar sources including flowers, fruits, seedpods, and honeydew in the laboratory and using plant-baited traps in the field. Next, we observed the rhythm of blood feeding versus sugar feeding activity of Ae. aegypti in vegetation and in open areas. Finally, we studied the effectiveness of spraying vegetation with ATSB on Ae. aegypti in sugar rich (lush vegetation) and in sugar poor (sparse vegetation) urban environments. Principal findings Male and female laboratory sugar feeding rates within 24 h, on 8 of 16 plants offered were over 80%. The survival rates of mosquitoes on several plant sources were nearly as long as that of controls maintained on sucrose solution. In the field, females were highly attracted to 11 of 20 sugar sources, and 8 of these were attractive to males. Peak periods of host attraction for blood-feeding and sugar feeding in open areas were nearly identical and occurred shortly after sunrise and around sunset. In shaded areas, the first sugar-seeking peak occurred between 11:30 and 12:30 while the second was from 16:30 to 17:30. In a 50-day field trial, ATSB significantly reduced mean numbers of landing / biting female Ae. aegypti in the two types of vegetation. At sugar poor sites, the mean pre-treatment catch of 20.51 females on day 14 was reduced 70-fold to 0.29 on day 50. At sugar rich sites, the mean pretreatment catch of 32.46 females on day 14 was reduced 10-fold to a mean of 3.20 females on day 50. Conclusions This is the first study to show how the vector Ae. aegypti depends on environmental resources of sugar for feeding and survival. The demonstration that Ae. aegypti populations rapidly collapsed after ATSB treatment, in both sugar rich and sugar poor environments, is strong evidence that Ae. aegypti is sugar-feeding frequently. Indeed, this study clearly demonstrates that Ae. aegypti mosquitoes depend on natural sugar resources, and a promising new method for vector control, ATSB, can be highly effective in the fight against Aedes-transmitted diseases.

AB - Background Current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including Zika, dengue, chikungunya, and yellow fever. Hence there is a growing public health challenge to identify the best new control tools to use against the vector Aedes aegypti. In this study, we investigated Ae. aegypti sugar feeding strategies in Bamako, Mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (ATSB). Methodology We determined the relative attraction of Ae. aegypti males and females to a variety of sugar sources including flowers, fruits, seedpods, and honeydew in the laboratory and using plant-baited traps in the field. Next, we observed the rhythm of blood feeding versus sugar feeding activity of Ae. aegypti in vegetation and in open areas. Finally, we studied the effectiveness of spraying vegetation with ATSB on Ae. aegypti in sugar rich (lush vegetation) and in sugar poor (sparse vegetation) urban environments. Principal findings Male and female laboratory sugar feeding rates within 24 h, on 8 of 16 plants offered were over 80%. The survival rates of mosquitoes on several plant sources were nearly as long as that of controls maintained on sucrose solution. In the field, females were highly attracted to 11 of 20 sugar sources, and 8 of these were attractive to males. Peak periods of host attraction for blood-feeding and sugar feeding in open areas were nearly identical and occurred shortly after sunrise and around sunset. In shaded areas, the first sugar-seeking peak occurred between 11:30 and 12:30 while the second was from 16:30 to 17:30. In a 50-day field trial, ATSB significantly reduced mean numbers of landing / biting female Ae. aegypti in the two types of vegetation. At sugar poor sites, the mean pre-treatment catch of 20.51 females on day 14 was reduced 70-fold to 0.29 on day 50. At sugar rich sites, the mean pretreatment catch of 32.46 females on day 14 was reduced 10-fold to a mean of 3.20 females on day 50. Conclusions This is the first study to show how the vector Ae. aegypti depends on environmental resources of sugar for feeding and survival. The demonstration that Ae. aegypti populations rapidly collapsed after ATSB treatment, in both sugar rich and sugar poor environments, is strong evidence that Ae. aegypti is sugar-feeding frequently. Indeed, this study clearly demonstrates that Ae. aegypti mosquitoes depend on natural sugar resources, and a promising new method for vector control, ATSB, can be highly effective in the fight against Aedes-transmitted diseases.

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