Frequent gain of chromosome 19 in megakaryoblastic leukemias detected by comparative genomic hybridization

Sara Alvarez, Donal MacGrogan, M. Jose Calasanz, Stephen D Nimer, Suresh C. Jhanwar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Acute megakaryocytic leukemia is a rare subtype of AML that is often difficult to diagnose; it is most commonly associated with Down syndrome in children. To identify chromosomal imbalances and rearrangements associated with acute megakaryocytic leukemia, we used G-banding, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) on a variety of primary patients' samples and leukemia cell lines. The most common abnormality was gain of chromosome 19 or arm 19q, which was detected by CGH in four of 12 (33.3%) primary samples and nine of 11 (81.8%) cell lines. In none of the primary samples was this abnormality detected by G-banding analysis. WCP was used to define further the nature of the chromosome 19 gain in the cell lines, which was found to be due to the presence of additional 19q material on marker chromosomes or to cryptic translocations involving 19q. The most common chromosomal loss-detected only in the cell lines-was deletion of chromosomal band 13q14, which was seen in six of 11 (54.5%) cell lines. Other recurrent changes included gains of 1p, 6p, 8q, 11q, 15q, 17q, and 21q and losses of 2, 4q, 5q, 7q, 9p, and 11p. Combining conventional and molecular cytogenetic analyses defined recurrent clonal chromosomal abnormalities, which will aid in the identification of critical genes that are abnormal in acute megakaryocytic leukemia cells.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalGenes Chromosomes and Cancer
Volume32
Issue number3
DOIs
StatePublished - Oct 22 2001
Externally publishedYes

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Chromosomes, Human, Pair 19
Comparative Genomic Hybridization
Leukemia, Megakaryoblastic, Acute
Leukemia
Cell Line
Chromosome Painting
Cytogenetic Analysis
Down Syndrome
Genetic Markers
Chromosome Aberrations
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Frequent gain of chromosome 19 in megakaryoblastic leukemias detected by comparative genomic hybridization. / Alvarez, Sara; MacGrogan, Donal; Calasanz, M. Jose; Nimer, Stephen D; Jhanwar, Suresh C.

In: Genes Chromosomes and Cancer, Vol. 32, No. 3, 22.10.2001, p. 285-293.

Research output: Contribution to journalArticle

Alvarez, Sara ; MacGrogan, Donal ; Calasanz, M. Jose ; Nimer, Stephen D ; Jhanwar, Suresh C. / Frequent gain of chromosome 19 in megakaryoblastic leukemias detected by comparative genomic hybridization. In: Genes Chromosomes and Cancer. 2001 ; Vol. 32, No. 3. pp. 285-293.
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