Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes

Gary E. Wise, Frances L. Shienvold, Robert Rubin, Clara Milikowski

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalTissue and Cell
Volume10
Issue number2
DOIs
StatePublished - Jan 1 1978

Fingerprint

erythrocytes
Erythrocytes
concanavalin A
Concanavalin A
cells
Freeze Etching
Concanavalin A Receptors
Adhesiveness
Cell Shape
adhesion
cell membranes
binding sites
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes. / Wise, Gary E.; Shienvold, Frances L.; Rubin, Robert; Milikowski, Clara.

In: Tissue and Cell, Vol. 10, No. 2, 01.01.1978, p. 219-225.

Research output: Contribution to journalArticle

Wise, Gary E. ; Shienvold, Frances L. ; Rubin, Robert ; Milikowski, Clara. / Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes. In: Tissue and Cell. 1978 ; Vol. 10, No. 2. pp. 219-225.
@article{6f326b19776242ca865bc361d6e6833f,
title = "Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes",
abstract = "Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.",
author = "Wise, {Gary E.} and Shienvold, {Frances L.} and Robert Rubin and Clara Milikowski",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/0040-8166(78)90019-8",
language = "English",
volume = "10",
pages = "219--225",
journal = "Tissue and Cell",
issn = "0040-8166",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes

AU - Wise, Gary E.

AU - Shienvold, Frances L.

AU - Rubin, Robert

AU - Milikowski, Clara

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.

AB - Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.

UR - http://www.scopus.com/inward/record.url?scp=0018188073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018188073&partnerID=8YFLogxK

U2 - 10.1016/0040-8166(78)90019-8

DO - 10.1016/0040-8166(78)90019-8

M3 - Article

C2 - 675662

AN - SCOPUS:0018188073

VL - 10

SP - 219

EP - 225

JO - Tissue and Cell

JF - Tissue and Cell

SN - 0040-8166

IS - 2

ER -