Abstract
Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.
| Original language | English |
|---|---|
| Pages (from-to) | 219-225 |
| Number of pages | 7 |
| Journal | Tissue and Cell |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1978 |
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ASJC Scopus subject areas
- Insect Science
- Cell Biology
Cite this
Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes. / Wise, Gary E.; Shienvold, Frances L.; Rubin, Robert; Milikowski, Clara.
In: Tissue and Cell, Vol. 10, No. 2, 01.01.1978, p. 219-225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Freeze-etch localization and distribution of concanavalin a in normal and irreversibly sickled erythrocytes
AU - Wise, Gary E.
AU - Shienvold, Frances L.
AU - Rubin, Robert
AU - Milikowski, Clara
PY - 1978/1/1
Y1 - 1978/1/1
N2 - Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.
AB - Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the etched face of the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution.
UR - http://www.scopus.com/inward/record.url?scp=0018188073&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018188073&partnerID=8YFLogxK
U2 - 10.1016/0040-8166(78)90019-8
DO - 10.1016/0040-8166(78)90019-8
M3 - Article
C2 - 675662
AN - SCOPUS:0018188073
VL - 10
SP - 219
EP - 225
JO - Tissue and Cell
JF - Tissue and Cell
SN - 0040-8166
IS - 2
ER -