Freeze-drying of solutions of serum albumin containing dimethylsulfoxide

Donald Greiff, Basil T. Doumas, Theodore I. Malinin, Billy W. Perry

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Although several publications have emphasized the inadvisability of drying biologic materials containing dimethylsulfoxide (DMSO) by sublimation of ice in vacuo, our studies showed a relationship to exist between the relative concentrations of serum albumin, human, and dimethylsulfoxide for successful or unsuccessful freeze-drying of albumin-DMSO solutions. The cycle of freeze-drying for the successful drying of an albumin-DMSO solution was a modification of the cycle used for the successful drying of suspensions of measles virus by sublimation of ice in vacuo. Using nuclear magnetic resonance spectroscopy, a strong, sharp signal for DMSO was obtained in preparations of freeze-dried albumin-DMSO solutions rehydrated with deuterium oxide, D2O.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalCryobiology
Volume13
Issue number2
DOIs
StatePublished - Apr 1976

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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