Free radical activity, antioxidant enzyme, and glutathione changes with muscle stretch injury in rabbits

Thomas Best, Russel Fiebig, David T. Corr, Stacey Brickson, Ji Lili

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The present study investigated changes in rate of free radical production, antioxidant enzyme activity, and glutathione status immediately after and 24 h after acute muscle stretch injury in 18 male New Zealand White rabbits. There was no change in free radical production in injured muscles, compared with noninjured controls, immediately after injury (time O; P = 0.782). However, at 24 h postinjury, there was a 25% increase in free radical production in the injured muscles. Overall, there was an interaction (time and treatment) effect (P = 0.005) for free radical production. Antioxidant enzyme activity demonstrated a treatment (injured vs. control) and interaction effect for both glutathione peroxidase (P = 0.015) and glutathione reductase (P = 0.041). There was no evidence of lipid peroxidation damage, as measured by muscle malondialdehyde content. An interaction effect occurred for both reduced glutathione (P = 0.008) and total glutathione (P = 0.015). Morphological analysis (hematoxylin and eosin staining) showed significant polymorphonuclear cell infiltration of the damaged region at 24 h postinjury. We conclude that acute mechanical muscle stretch injury results in increased free radical production within 24 h after injury. Antioxidant enzyme and glutathione systems also appear to be affected during this early postinjury period.

Original languageEnglish (US)
Pages (from-to)74-82
Number of pages9
JournalJournal of Applied Physiology
Volume87
Issue number1
StatePublished - Jul 1 1999
Externally publishedYes

Fingerprint

Free Radicals
Glutathione
Antioxidants
Rabbits
Muscles
Wounds and Injuries
Enzymes
Glutathione Reductase
Hematoxylin
Eosine Yellowish-(YS)
Glutathione Peroxidase
Malondialdehyde
Lipid Peroxidation
Staining and Labeling

Keywords

  • Free radicals
  • Muscle stretch injury

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Free radical activity, antioxidant enzyme, and glutathione changes with muscle stretch injury in rabbits. / Best, Thomas; Fiebig, Russel; Corr, David T.; Brickson, Stacey; Lili, Ji.

In: Journal of Applied Physiology, Vol. 87, No. 1, 01.07.1999, p. 74-82.

Research output: Contribution to journalArticle

Best, Thomas ; Fiebig, Russel ; Corr, David T. ; Brickson, Stacey ; Lili, Ji. / Free radical activity, antioxidant enzyme, and glutathione changes with muscle stretch injury in rabbits. In: Journal of Applied Physiology. 1999 ; Vol. 87, No. 1. pp. 74-82.
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