Free fatty acids and energy metabolites in ischemic cerebral cortex with noradrenaline depletion

S. Yoshida, S. I. Harik, R. Busto, M. Santiso, E. Martinez, Myron Ginsberg

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We tested whether cerebral noradrenaline (NA) may play a central role in mediating the increased production of free fatty acids (FFAs) during cerebral ischemia. Levels of FFAs, cyclic AMP, and NA, as well as ATP, ADP, and AMP, were measured in cerebral cortex during decapitation ischemia in rats 2 weeks after unilateral locus ceruleus lesion. Comparisons were made between the results obtained from the contralateral cortex with normal NA content and the NA-depleted ipsilateral cortex. Although NA depletion was associated with a diminished transient rise of cyclic AMP in response to ischemia, it failed to influence the magnitude of FFA increase or the decline of energy state within the 15-min period of ischemia. A more than twofold increase of total FFAs (sum of palmitic, stearic, oleic, arachidonic, and docosahexaenoic acids) was observed in both hemispheres at 1 min after decapitation, when energy failure became manifest. The increased production of FFAs continued throughout the 15 min of ischemia, with a preferential rise in the levels of stearic and arachidonic acids. There was an inverse correlation between FFA levels and total adenylate pool. The results do not support a major role for NA and cyclic AMP in increasing cortical FFAs during complete ischemia. Instead, they are consistent with the view that impaired oxidative phosphorylation activates deacylating enzymes. Disturbance of reacylation due to energy depletion is probably another factor contributing to the continuous increase of FFAs during prolonged ischemia.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalJournal of Neurochemistry
Volume42
Issue number3
StatePublished - Apr 26 1984

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Metabolites
Nonesterified Fatty Acids
Cerebral Cortex
Norepinephrine
Ischemia
Cyclic AMP
Decapitation
Stearic Acids
Arachidonic Acids
Locus Coeruleus
Docosahexaenoic Acids
Oxidative Phosphorylation
Adenosine Monophosphate
Brain Ischemia
Adenosine Diphosphate
Electron energy levels
Rats
Adenosine Triphosphate
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Yoshida, S., Harik, S. I., Busto, R., Santiso, M., Martinez, E., & Ginsberg, M. (1984). Free fatty acids and energy metabolites in ischemic cerebral cortex with noradrenaline depletion. Journal of Neurochemistry, 42(3), 711-717.

Free fatty acids and energy metabolites in ischemic cerebral cortex with noradrenaline depletion. / Yoshida, S.; Harik, S. I.; Busto, R.; Santiso, M.; Martinez, E.; Ginsberg, Myron.

In: Journal of Neurochemistry, Vol. 42, No. 3, 26.04.1984, p. 711-717.

Research output: Contribution to journalArticle

Yoshida, S, Harik, SI, Busto, R, Santiso, M, Martinez, E & Ginsberg, M 1984, 'Free fatty acids and energy metabolites in ischemic cerebral cortex with noradrenaline depletion', Journal of Neurochemistry, vol. 42, no. 3, pp. 711-717.
Yoshida, S. ; Harik, S. I. ; Busto, R. ; Santiso, M. ; Martinez, E. ; Ginsberg, Myron. / Free fatty acids and energy metabolites in ischemic cerebral cortex with noradrenaline depletion. In: Journal of Neurochemistry. 1984 ; Vol. 42, No. 3. pp. 711-717.
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