Free circulating DNA as a biomarker of prostate cancer: Comparison of quantitation methods

Kavitha Ramachandran, Carl G. Speer, Stephanie Fiddy, Isildinha M. Reis, Rakesh Singal

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Aim: To identify a simpler method of free circulating DNA (fcDNA) quantitation that may improve the specificity of the prostate cancer prostate-specific antigen (PSA) screening test. Materials and Methods: The patient group consisted of 241 men with elevated PSA/abnormal digital rectal exam (DRE), undergoing prostate biopsy. Serum fcDNA levels were measured by UV absorbance and PicoGreen. Results were compared to previously published quantitative polymerase chain reaction (qPCR) data. Results: We found that levels of fcDNA measured by PicoGreen correlated well with those measured by qPCR (r=0.8552). In the patient group with PSA >4 to 10 ng/ml, those with fcDNA (PicoGreen) >53.1 ng/ml were at increased risk for prostate cancer compared to those with fcDNA ≤53.1 ng/ml. Moreover, we found that measuring fcDNA levels by PicoGreen does not compromise the negative predictive value, accuracy or specificity of the qPCR fcDNA test. Conclusion: If validated in larger studies, PicoGreen quantitation of fcDNA could serve as a simple method to aid in prostate cancer diagnosis.

Original languageEnglish (US)
Pages (from-to)4521-4530
Number of pages10
JournalAnticancer research
Volume33
Issue number10
StatePublished - Oct 1 2013

Keywords

  • Biomarker
  • Cell-free DNA
  • PicoGreen
  • Prostate cancer
  • Quantitative PCR
  • Screening

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Free circulating DNA as a biomarker of prostate cancer: Comparison of quantitation methods'. Together they form a unique fingerprint.

Cite this