FOXF2 is required for cochlear development in humans and mice

Guney Bademci, Clemer Abad, Armagan Incesulu, Fahed Elian, Azadeh Reyahi, Oscar Diaz-Horta, Filiz B. Cengiz, Claire J. Sineni, Serhat Seyhan, Emine Ikbal Atli, Hikmet Basmak, Selma Demir, Ali Moussavi Nik, Tim Footz, Shengru Guo, Duygu Duman, Suat Fitoz, Hakan Gurkan, Susan H Blanton, Michael A. WalterPeter Carlsson, Katherina Walz, Mustafa Tekin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Molecular mechanisms governing the development of the human cochlea remain largely unknown. Through genome sequencing, we identified a homozygous FOXF2 variant c.325A>T (p.I109F) in a child with profound sensorineural hearing loss (SNHL) associated with incomplete partition type I anomaly of the cochlea. This variant is not found in public databases or in over 1000 ethnicity-matched control individuals. I109 is a highly conserved residue in the forkhead box (Fox) domain of FOXF2, a member of the Fox protein family of transcription factors that regulate the expression of genes involved in embryogenic development as well as adult life. Our in vitro studies show that the half-life of mutant FOXF2 is reduced compared to that of wild type. Foxf2 is expressed in the cochlea of developing and adult mice. The mouse knockout of Foxf2 shows shortened and malformed cochleae, in addition to altered shape of hair cells with innervation and planar cell polarity defects. Expressions of Eya1 and Pax3, genes essential for cochlear development, are reduced in the cochleae of Foxf2 knockout mice. We conclude that FOXF2 plays a major role in cochlear development and its dysfunction leads to SNHL and developmental anomalies of the cochlea in humans and mice.

Original languageEnglish (US)
Pages (from-to)1286-1297
Number of pages12
JournalHuman molecular genetics
Volume28
Issue number8
DOIs
StatePublished - Apr 15 2019

Fingerprint

Cochlea
Human Development
Sensorineural Hearing Loss
Knockout Mice
Cell Polarity
Cell Shape
Essential Genes
Half-Life
Transcription Factors
Genome
Databases
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Bademci, G., Abad, C., Incesulu, A., Elian, F., Reyahi, A., Diaz-Horta, O., ... Tekin, M. (2019). FOXF2 is required for cochlear development in humans and mice. Human molecular genetics, 28(8), 1286-1297. https://doi.org/10.1093/hmg/ddy431

FOXF2 is required for cochlear development in humans and mice. / Bademci, Guney; Abad, Clemer; Incesulu, Armagan; Elian, Fahed; Reyahi, Azadeh; Diaz-Horta, Oscar; Cengiz, Filiz B.; Sineni, Claire J.; Seyhan, Serhat; Atli, Emine Ikbal; Basmak, Hikmet; Demir, Selma; Nik, Ali Moussavi; Footz, Tim; Guo, Shengru; Duman, Duygu; Fitoz, Suat; Gurkan, Hakan; Blanton, Susan H; Walter, Michael A.; Carlsson, Peter; Walz, Katherina; Tekin, Mustafa.

In: Human molecular genetics, Vol. 28, No. 8, 15.04.2019, p. 1286-1297.

Research output: Contribution to journalArticle

Bademci, G, Abad, C, Incesulu, A, Elian, F, Reyahi, A, Diaz-Horta, O, Cengiz, FB, Sineni, CJ, Seyhan, S, Atli, EI, Basmak, H, Demir, S, Nik, AM, Footz, T, Guo, S, Duman, D, Fitoz, S, Gurkan, H, Blanton, SH, Walter, MA, Carlsson, P, Walz, K & Tekin, M 2019, 'FOXF2 is required for cochlear development in humans and mice', Human molecular genetics, vol. 28, no. 8, pp. 1286-1297. https://doi.org/10.1093/hmg/ddy431
Bademci G, Abad C, Incesulu A, Elian F, Reyahi A, Diaz-Horta O et al. FOXF2 is required for cochlear development in humans and mice. Human molecular genetics. 2019 Apr 15;28(8):1286-1297. https://doi.org/10.1093/hmg/ddy431
Bademci, Guney ; Abad, Clemer ; Incesulu, Armagan ; Elian, Fahed ; Reyahi, Azadeh ; Diaz-Horta, Oscar ; Cengiz, Filiz B. ; Sineni, Claire J. ; Seyhan, Serhat ; Atli, Emine Ikbal ; Basmak, Hikmet ; Demir, Selma ; Nik, Ali Moussavi ; Footz, Tim ; Guo, Shengru ; Duman, Duygu ; Fitoz, Suat ; Gurkan, Hakan ; Blanton, Susan H ; Walter, Michael A. ; Carlsson, Peter ; Walz, Katherina ; Tekin, Mustafa. / FOXF2 is required for cochlear development in humans and mice. In: Human molecular genetics. 2019 ; Vol. 28, No. 8. pp. 1286-1297.
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