Fourier ptychographic microscopy for rapid, high-resolution imaging of circulating tumor cells enriched by microfiltration

Anthony Williams, Jaebum Chung, Changhuei Yang, Richard J. Cote

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Examining the hematogenous compartment for evidence of metastasis has increased significantly within the oncology research community in recent years, due to the development of technologies aimed at the enrichment of circulating tumor cells (CTCs), the subpopulation of primary tumor cells that gain access to the circulatory system and are responsible for colonization at distant sites. In contrast to other technologies, filtration-based CTC enrichment, which exploits differences in size between larger tumor cells and surrounding smaller, non-tumor blood cells, has the potential to improve CTC characterization through isolation of tumor cell populations with greater molecular heterogeneity. However, microscopic analysis of uneven filtration surfaces containing CTCs is laborious, time-consuming, and inconsistent, preventing widespread use of filtration-based enrichment technologies. Here, integrated with a microfiltration-based CTC and rare cell enrichment device we have previously described, we present a protocol for Fourier Ptychographic Microscopy (FPM), a method that, unlike many automated imaging platforms, produces high-speed, high-resolution images that can be digitally refocused, allowing users to observe objects of interest present on multiple focal planes within the same image frame. The development of a cost-effective and high-throughput CTC analysis system for filtration-based enrichment technologies could have profound clinical implications for improved CTC detection and analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-117
Number of pages11
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1634
ISSN (Print)1064-3745

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Keywords

  • Circulating tumor cells
  • Filtration-based CTC enrichment
  • Fourier ptychographic microscopy
  • Metastasis
  • Single-cell imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Williams, A., Chung, J., Yang, C., & Cote, R. J. (2017). Fourier ptychographic microscopy for rapid, high-resolution imaging of circulating tumor cells enriched by microfiltration. In Methods in Molecular Biology (pp. 107-117). (Methods in Molecular Biology; Vol. 1634). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7144-2_8