Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis

Anthony Williams, Jaebum Chung, Xiaoze Ou, Guoan Zheng, Siddarth Rawal, Zheng Ao, Ram Datar, Changhuei Yang, Richard J Cote

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are recognized as a candidate biomarker with strong prognostic and predictive potential in metastatic disease. Filtration-based enrichment technologies have been used for CTC characterization, and our group has previously developed a membrane microfilter device that demonstrates efficacy in model systems and clinical blood samples. However, uneven filtration surfaces make the use of standard microscopic techniques a difficult task, limiting the performance of automated imaging using commercially available technologies. Here, we report the use of Fourier ptychographic microscopy (FPM) to tackle this challenge. Employing this method, we were able to obtain high-resolution color images, including amplitude and phase, of the microfilter samples over large areas. FPM's ability to perform digital refocusing on complex images is particularly useful in this setting as, in contrast to other imaging platforms, we can focus samples on multiple focal planes within the same frame despite surface unevenness. In model systems, FPM demonstrates high image quality, efficiency, and consistency in detection of tumor cells when comparing corresponding microfilter samples to standard microscopy with high correlation (R2 = 0.99932). Based on these results, we believe that FPM will have important implications for improved, high throughput, filtration-based CTC analysis, and, more generally, image analysis of uneven surfaces.

Original languageEnglish (US)
Article number066007
JournalJournal of Biomedical Optics
Volume19
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Circulating Neoplastic Cells
enumeration
Tumors
Microscopy
Microscopic examination
tumors
Cells
microscopy
Technology
Imaging techniques
Aptitude
biomarkers
Task Performance and Analysis
Biomarkers
image analysis
Image analysis
Image quality
blood
Blood
platforms

Keywords

  • circulating tumor cells
  • Fourier ptychographic microscopy
  • metastasis
  • microfilter device

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Medicine(all)

Cite this

Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis. / Williams, Anthony; Chung, Jaebum; Ou, Xiaoze; Zheng, Guoan; Rawal, Siddarth; Ao, Zheng; Datar, Ram; Yang, Changhuei; Cote, Richard J.

In: Journal of Biomedical Optics, Vol. 19, No. 6, 066007, 2014.

Research output: Contribution to journalArticle

Williams, Anthony ; Chung, Jaebum ; Ou, Xiaoze ; Zheng, Guoan ; Rawal, Siddarth ; Ao, Zheng ; Datar, Ram ; Yang, Changhuei ; Cote, Richard J. / Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis. In: Journal of Biomedical Optics. 2014 ; Vol. 19, No. 6.
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