Four novel U RNAs are encoded by a herpesvirus

Susanna I. Lee, Shridhara C.S. Murthy, John J. Trimble, Ronald C. Desrosiers, Joan A. Steitz

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Marmoset T lymphocytes transformed by herpesvirus saimiri contain the first virally encoded U RNAs (called HSURs) to be identified. HSURs assemble into small nuclear ribonucleoproteins of low abundance (≤ 2 × 104 copies/cell). They bind proteins with Sm determinants and acquire a 5′ trimethylguanosine cap structure. The sequences of HSUR 1 (143 nucleotides), HSUR 2 (115 nucleotides), HSUR 3 (76 nucleotides), and HSUR 4 (106 nucleotides) are related to each other but are distinct from any previously characterized cellular U RNA. The viral genes encoding the HSURs possess conserved enhancer, promoter, and 3′ end formation signals unique to U RNA genes. HSUR 1 and HSUR 2 have a similar 5′ end sequence that exhibits perfect complementarity to the highly conserved AAUAAA polyadenylation signal. Oligonucleotide directed RNAase H degradation indicates that this 5′ end region is available for base pairing interactions within the HSUR 1 and HSUR 2 snRNP particles.

Original languageEnglish (US)
Pages (from-to)599-607
Number of pages9
JournalCell
Volume54
Issue number5
DOIs
StatePublished - Aug 26 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lee, S. I., Murthy, S. C. S., Trimble, J. J., Desrosiers, R. C., & Steitz, J. A. (1988). Four novel U RNAs are encoded by a herpesvirus. Cell, 54(5), 599-607. https://doi.org/10.1016/S0092-8674(88)80004-7