TY - JOUR
T1 - Four novel U RNAs are encoded by a herpesvirus
AU - Lee, Susanna I.
AU - Murthy, Shridhara C.S.
AU - Trimble, John J.
AU - Desrosiers, Ronald C.
AU - Steitz, Joan A.
N1 - Funding Information:
We thank Dan Gewirth, Greg Howe, and Kazimierz Tyc for helpful advice and discussion throughout the course of this work. This investigation was sponsored by NIH grants GM26154 and CA16038 to J. A. S., CA31363 to R. C. D., GM07205 to the Medical Scientist Training Program, Yale Medical School, and RR00168 to NERPRC. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore he hereby marked "advertisement" in accordance with 16 U.S.C. Section 1734 solely to indicate this fact.
PY - 1988/8/26
Y1 - 1988/8/26
N2 - Marmoset T lymphocytes transformed by herpesvirus saimiri contain the first virally encoded U RNAs (called HSURs) to be identified. HSURs assemble into small nuclear ribonucleoproteins of low abundance (≤ 2 × 104 copies/cell). They bind proteins with Sm determinants and acquire a 5′ trimethylguanosine cap structure. The sequences of HSUR 1 (143 nucleotides), HSUR 2 (115 nucleotides), HSUR 3 (76 nucleotides), and HSUR 4 (106 nucleotides) are related to each other but are distinct from any previously characterized cellular U RNA. The viral genes encoding the HSURs possess conserved enhancer, promoter, and 3′ end formation signals unique to U RNA genes. HSUR 1 and HSUR 2 have a similar 5′ end sequence that exhibits perfect complementarity to the highly conserved AAUAAA polyadenylation signal. Oligonucleotide directed RNAase H degradation indicates that this 5′ end region is available for base pairing interactions within the HSUR 1 and HSUR 2 snRNP particles.
AB - Marmoset T lymphocytes transformed by herpesvirus saimiri contain the first virally encoded U RNAs (called HSURs) to be identified. HSURs assemble into small nuclear ribonucleoproteins of low abundance (≤ 2 × 104 copies/cell). They bind proteins with Sm determinants and acquire a 5′ trimethylguanosine cap structure. The sequences of HSUR 1 (143 nucleotides), HSUR 2 (115 nucleotides), HSUR 3 (76 nucleotides), and HSUR 4 (106 nucleotides) are related to each other but are distinct from any previously characterized cellular U RNA. The viral genes encoding the HSURs possess conserved enhancer, promoter, and 3′ end formation signals unique to U RNA genes. HSUR 1 and HSUR 2 have a similar 5′ end sequence that exhibits perfect complementarity to the highly conserved AAUAAA polyadenylation signal. Oligonucleotide directed RNAase H degradation indicates that this 5′ end region is available for base pairing interactions within the HSUR 1 and HSUR 2 snRNP particles.
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U2 - 10.1016/S0092-8674(88)80004-7
DO - 10.1016/S0092-8674(88)80004-7
M3 - Article
C2 - 2842058
AN - SCOPUS:0023724428
VL - 54
SP - 599
EP - 607
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -