Formulation and optimization of mitochondria-targeted polymeric nanoparticles

Sean Marrache, Rakesh K. Pathak, Shanta Dhar

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations


Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria- targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(DL -lactide- co -glycolide)- b -polyethylene glycol-TPP and a nontargeted poly(DL -lactide- co - glycolide)- b -polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.

Original languageEnglish (US)
Title of host publicationManipulating Mitochondrial Function
PublisherSpringer New York
Number of pages10
ISBN (Electronic)9781493922888
ISBN (Print)9781493922871
StatePublished - Jan 29 2015
Externally publishedYes


  • Biodegradable polymeric nanoparticles
  • Mitochondria
  • Nanoprecipitation
  • Poly(DL-lactide-co-glycolide)
  • Polymer blending
  • Triphenylphosphonium cation

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Marrache, S., Pathak, R. K., & Dhar, S. (2015). Formulation and optimization of mitochondria-targeted polymeric nanoparticles. In Manipulating Mitochondrial Function (Vol. 2, pp. 103-112). Springer New York.