Formulation and optimization of mitochondria-targeted polymeric nanoparticles

Sean Marrache, Rakesh K. Pathak, Shanta Dhar

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria- targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(DL -lactide- co -glycolide)- b -polyethylene glycol-TPP and a nontargeted poly(DL -lactide- co - glycolide)- b -polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.

Original languageEnglish (US)
Title of host publicationManipulating Mitochondrial Function
PublisherSpringer New York
Pages103-112
Number of pages10
Volume2
ISBN (Electronic)9781493922888
ISBN (Print)9781493922871
DOIs
StatePublished - Jan 29 2015
Externally publishedYes

Fingerprint

Nanoparticles
Mitochondria
Polymers
Polyglactin 910
Cations
Mitochondrial Membranes
Technology
Pharmaceutical Preparations
poly(lactide)
Therapeutics

Keywords

  • Biodegradable polymeric nanoparticles
  • Mitochondria
  • Nanoprecipitation
  • Poly(DL-lactide-co-glycolide)
  • Polymer blending
  • Triphenylphosphonium cation

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Marrache, S., Pathak, R. K., & Dhar, S. (2015). Formulation and optimization of mitochondria-targeted polymeric nanoparticles. In Manipulating Mitochondrial Function (Vol. 2, pp. 103-112). Springer New York. https://doi.org/10.1007/978-1-4939-2288-8_8

Formulation and optimization of mitochondria-targeted polymeric nanoparticles. / Marrache, Sean; Pathak, Rakesh K.; Dhar, Shanta.

Manipulating Mitochondrial Function. Vol. 2 Springer New York, 2015. p. 103-112.

Research output: Chapter in Book/Report/Conference proceedingChapter

Marrache, S, Pathak, RK & Dhar, S 2015, Formulation and optimization of mitochondria-targeted polymeric nanoparticles. in Manipulating Mitochondrial Function. vol. 2, Springer New York, pp. 103-112. https://doi.org/10.1007/978-1-4939-2288-8_8
Marrache S, Pathak RK, Dhar S. Formulation and optimization of mitochondria-targeted polymeric nanoparticles. In Manipulating Mitochondrial Function. Vol. 2. Springer New York. 2015. p. 103-112 https://doi.org/10.1007/978-1-4939-2288-8_8
Marrache, Sean ; Pathak, Rakesh K. ; Dhar, Shanta. / Formulation and optimization of mitochondria-targeted polymeric nanoparticles. Manipulating Mitochondrial Function. Vol. 2 Springer New York, 2015. pp. 103-112
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