Formulation and optimization of mitochondria-targeted polymeric nanoparticles

Sean Marrache, Rakesh K. Pathak, Shanta Dhar

Research output: Contribution to journalArticle

Abstract

Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria- targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(DL -lactide- co -glycolide)- b -polyethylene glycol-TPP and a nontargeted poly(DL -lactide- co - glycolide)- b -polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalMethods in Molecular Biology
Volume1265
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Biodegradable polymeric nanoparticles
  • Mitochondria
  • Nanoprecipitation
  • Poly(DL -lactide- co -glycolide)
  • Polymer blending
  • Triphenylphosphonium cation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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