Abstract
Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria- targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(DL -lactide- co -glycolide)- b -polyethylene glycol-TPP and a nontargeted poly(DL -lactide- co - glycolide)- b -polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.
Original language | English (US) |
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Pages (from-to) | 103-112 |
Number of pages | 10 |
Journal | Methods in Molecular Biology |
Volume | 1265 |
DOIs | |
State | Published - Jan 1 2015 |
Externally published | Yes |
Keywords
- Biodegradable polymeric nanoparticles
- Mitochondria
- Nanoprecipitation
- Poly(DL -lactide- co -glycolide)
- Polymer blending
- Triphenylphosphonium cation
ASJC Scopus subject areas
- Molecular Biology
- Genetics