Forms of g protein β subunit gβ5 expressed in retina

J. L. Cabrera, F. De Freitag, J. Chen, Vladlen Z Slepak

Research output: Contribution to journalArticle

Abstract

Gβ5 is the only G protein β-subunit specifically expressed in neuronal cells. A splice variant of Gβ5 which is longer than Gβ5 by 40 amino acids (Gβ5L) is only expressed in photoreceptors (1). Here, we studied the localization of the two forms in more detail and investigated its dependence on light. Immunohistochemical staining of sections of dark-adapted mouse retinas revealed Gβ5 immunoreactivity in the inner (INL) and outer (ONL) nuclear layers as well as in the outer segments (OS). In the light-adapted tissue, the staining in the ONL and OS was dramatically reduced. Intact bovine OS, purified by ultracentrifugation, contained only GβSL, while Gβ5 (short) was mainly found in the soluble lysate of retina. A small fraction of Gβ5 was also associated with membranes that did not contain rhodopsin and most likely originated from non-photoreceptor cells. To begin the identification of other members of the Gβ5-mediated pathway, we purified the native complex of soluble Gβ5 to near homogeneity. Gβ5 is coupled to a 10 kDa protein, apparently a Gy subunit. This Gy is, however, distinct from the y subunit of transducin (Gyl) as well as from Gy2, Gv3, Gy5 and Gy7. Unexpectedly, we found that the native complex does not contain any known Get subunits or phosducin, but rather a yet unidentified 65 kDa protein. Identification of the two Gβ5-associated proteins is now in progress. 1. Watson, J.A., Aragay, A.M., Siepak, V.Z., Simon, M.I. A novel form of the G protein βsubunit Gβ5 specifically expressed in vertebrate retina (1996) J. Biol. Chem.. 271, 28154. & American Heart Association (9603008); Stanley Glaser Foundation; # Researh to Prevent Blindness; Ruth and Milton Steinbach Fund.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number8
StatePublished - Dec 1 1998
Externally publishedYes

Fingerprint

Protein Subunits
protein subunits
retina
Retina
G-proteins
GTP-Binding Proteins
Transducin
Staining and Labeling
Light
rhodopsin
Proteins
Rhodopsin
ultracentrifugation
proteins
blindness
Ultracentrifugation
Blindness
photoreceptors
Vertebrates
neurons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cabrera, J. L., De Freitag, F., Chen, J., & Slepak, V. Z. (1998). Forms of g protein β subunit gβ5 expressed in retina. FASEB Journal, 12(8).

Forms of g protein β subunit gβ5 expressed in retina. / Cabrera, J. L.; De Freitag, F.; Chen, J.; Slepak, Vladlen Z.

In: FASEB Journal, Vol. 12, No. 8, 01.12.1998.

Research output: Contribution to journalArticle

Cabrera, JL, De Freitag, F, Chen, J & Slepak, VZ 1998, 'Forms of g protein β subunit gβ5 expressed in retina', FASEB Journal, vol. 12, no. 8.
Cabrera JL, De Freitag F, Chen J, Slepak VZ. Forms of g protein β subunit gβ5 expressed in retina. FASEB Journal. 1998 Dec 1;12(8).
Cabrera, J. L. ; De Freitag, F. ; Chen, J. ; Slepak, Vladlen Z. / Forms of g protein β subunit gβ5 expressed in retina. In: FASEB Journal. 1998 ; Vol. 12, No. 8.
@article{cb102dff25e34bb4a9432eecb009874e,
title = "Forms of g protein β subunit gβ5 expressed in retina",
abstract = "Gβ5 is the only G protein β-subunit specifically expressed in neuronal cells. A splice variant of Gβ5 which is longer than Gβ5 by 40 amino acids (Gβ5L) is only expressed in photoreceptors (1). Here, we studied the localization of the two forms in more detail and investigated its dependence on light. Immunohistochemical staining of sections of dark-adapted mouse retinas revealed Gβ5 immunoreactivity in the inner (INL) and outer (ONL) nuclear layers as well as in the outer segments (OS). In the light-adapted tissue, the staining in the ONL and OS was dramatically reduced. Intact bovine OS, purified by ultracentrifugation, contained only GβSL, while Gβ5 (short) was mainly found in the soluble lysate of retina. A small fraction of Gβ5 was also associated with membranes that did not contain rhodopsin and most likely originated from non-photoreceptor cells. To begin the identification of other members of the Gβ5-mediated pathway, we purified the native complex of soluble Gβ5 to near homogeneity. Gβ5 is coupled to a 10 kDa protein, apparently a Gy subunit. This Gy is, however, distinct from the y subunit of transducin (Gyl) as well as from Gy2, Gv3, Gy5 and Gy7. Unexpectedly, we found that the native complex does not contain any known Get subunits or phosducin, but rather a yet unidentified 65 kDa protein. Identification of the two Gβ5-associated proteins is now in progress. 1. Watson, J.A., Aragay, A.M., Siepak, V.Z., Simon, M.I. A novel form of the G protein βsubunit Gβ5 specifically expressed in vertebrate retina (1996) J. Biol. Chem.. 271, 28154. & American Heart Association (9603008); Stanley Glaser Foundation; # Researh to Prevent Blindness; Ruth and Milton Steinbach Fund.",
author = "Cabrera, {J. L.} and {De Freitag}, F. and J. Chen and Slepak, {Vladlen Z}",
year = "1998",
month = "12",
day = "1",
language = "English",
volume = "12",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "8",

}

TY - JOUR

T1 - Forms of g protein β subunit gβ5 expressed in retina

AU - Cabrera, J. L.

AU - De Freitag, F.

AU - Chen, J.

AU - Slepak, Vladlen Z

PY - 1998/12/1

Y1 - 1998/12/1

N2 - Gβ5 is the only G protein β-subunit specifically expressed in neuronal cells. A splice variant of Gβ5 which is longer than Gβ5 by 40 amino acids (Gβ5L) is only expressed in photoreceptors (1). Here, we studied the localization of the two forms in more detail and investigated its dependence on light. Immunohistochemical staining of sections of dark-adapted mouse retinas revealed Gβ5 immunoreactivity in the inner (INL) and outer (ONL) nuclear layers as well as in the outer segments (OS). In the light-adapted tissue, the staining in the ONL and OS was dramatically reduced. Intact bovine OS, purified by ultracentrifugation, contained only GβSL, while Gβ5 (short) was mainly found in the soluble lysate of retina. A small fraction of Gβ5 was also associated with membranes that did not contain rhodopsin and most likely originated from non-photoreceptor cells. To begin the identification of other members of the Gβ5-mediated pathway, we purified the native complex of soluble Gβ5 to near homogeneity. Gβ5 is coupled to a 10 kDa protein, apparently a Gy subunit. This Gy is, however, distinct from the y subunit of transducin (Gyl) as well as from Gy2, Gv3, Gy5 and Gy7. Unexpectedly, we found that the native complex does not contain any known Get subunits or phosducin, but rather a yet unidentified 65 kDa protein. Identification of the two Gβ5-associated proteins is now in progress. 1. Watson, J.A., Aragay, A.M., Siepak, V.Z., Simon, M.I. A novel form of the G protein βsubunit Gβ5 specifically expressed in vertebrate retina (1996) J. Biol. Chem.. 271, 28154. & American Heart Association (9603008); Stanley Glaser Foundation; # Researh to Prevent Blindness; Ruth and Milton Steinbach Fund.

AB - Gβ5 is the only G protein β-subunit specifically expressed in neuronal cells. A splice variant of Gβ5 which is longer than Gβ5 by 40 amino acids (Gβ5L) is only expressed in photoreceptors (1). Here, we studied the localization of the two forms in more detail and investigated its dependence on light. Immunohistochemical staining of sections of dark-adapted mouse retinas revealed Gβ5 immunoreactivity in the inner (INL) and outer (ONL) nuclear layers as well as in the outer segments (OS). In the light-adapted tissue, the staining in the ONL and OS was dramatically reduced. Intact bovine OS, purified by ultracentrifugation, contained only GβSL, while Gβ5 (short) was mainly found in the soluble lysate of retina. A small fraction of Gβ5 was also associated with membranes that did not contain rhodopsin and most likely originated from non-photoreceptor cells. To begin the identification of other members of the Gβ5-mediated pathway, we purified the native complex of soluble Gβ5 to near homogeneity. Gβ5 is coupled to a 10 kDa protein, apparently a Gy subunit. This Gy is, however, distinct from the y subunit of transducin (Gyl) as well as from Gy2, Gv3, Gy5 and Gy7. Unexpectedly, we found that the native complex does not contain any known Get subunits or phosducin, but rather a yet unidentified 65 kDa protein. Identification of the two Gβ5-associated proteins is now in progress. 1. Watson, J.A., Aragay, A.M., Siepak, V.Z., Simon, M.I. A novel form of the G protein βsubunit Gβ5 specifically expressed in vertebrate retina (1996) J. Biol. Chem.. 271, 28154. & American Heart Association (9603008); Stanley Glaser Foundation; # Researh to Prevent Blindness; Ruth and Milton Steinbach Fund.

UR - http://www.scopus.com/inward/record.url?scp=33749110392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749110392&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749110392

VL - 12

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 8

ER -