Formation of high affinity IL-2 receptors is dependent on a nonligand binding region of the a subunit

Nancy S. Gutgsell, Thomas Malek

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The IL-2R is composed of three sub-units, α, β, and γ, each of which individually contributes to binding IL-2 in the high affinity IL-2R. The molecular mechanism by which the high affinity IL-2R assembles has not been established. Previous studies have shown that human IL-2R subunits form high affinity hybrid IL-2R when mixed with appropriate mouse IL-2R subunits, but the efficiency of this process is not known. In this study, we produced stable cell lines that expressed hybrid human/mouse IL-2R, comprised of heterologous α- and β-chains, to determine the contribution of individual receptor subunits in stabilizing the high affinity IL-2R. Quantitative ligand binding studies and FACS analysis were performed for EL4J cells that expressed hybrid mouse/human IL-2R, and these data were compared with those of cells that expressed homologous mouse IL-2R subunits. EL4J cells that expressed human β/mouse α formed high affinity IL-2R, comparable with cells that express homologous mouse α and β subunits. In contrast, EL4J cells that expressed homologous mouse IL-2R contained an approximately 10-fold higher level of high affinity IL-2R than did EL4J cells that expressed heterologous human α/mouse β IL-2R. This difference was not accounted for by lower levels of human α-chains in these cell lines or lower expression of the mouse IL-2R β or γ subunits. These results suggest that amino acid sequences within the α subunit, distinct from the IL-2 binding site, are important in the assembly and stabilization of the high affinity IL-2R.

Original languageEnglish
Pages (from-to)3899-3907
Number of pages9
JournalJournal of Immunology
Volume153
Issue number9
StatePublished - Nov 1 1994

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Interleukin-2 Receptors
Interleukin-2
Cell Line
Hybrid Cells
Amino Acid Sequence
Binding Sites
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Formation of high affinity IL-2 receptors is dependent on a nonligand binding region of the a subunit. / Gutgsell, Nancy S.; Malek, Thomas.

In: Journal of Immunology, Vol. 153, No. 9, 01.11.1994, p. 3899-3907.

Research output: Contribution to journalArticle

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